Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.)

• Published in: Parasites & vectors Vol. 4; no. 1; p. 237
• Main Authors: Gaio, Analiz de Oliveira, Rodrigues, Rivea CC, do Nascimento, Cássio, Secundino, Nagila FC, Lemos, Francisco JA, Pimenta, Paulo FP, Monesi, Nadia
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 20.12.2011; BioMed Central Ltd; BioMed Central; BMC
• Subjects: adults; Aedes - growth & development; Aedes - microbiology; Aedes aegypti; Animals; Apis mellifera; Bacteria; Bacteria - classification; Bacteria - genetics; Bees - microbiology; Bradysia; Checkerboard DNA-DNA hybridization; DNA; DNA, Bacterial - genetics; Drosophila melanogaster; Drosophila melanogaster - microbiology; Entomology - methods; Genetic aspects; insects; Larva - microbiology; larvae; Lutzomyia longipalpis; microbial detection; midgut; nucleic acid hybridization; Nucleic Acid Hybridization - methods; Physiological aspects; plate count; Psychodidae - microbiology; Pupa - microbiology; pupae; Sensitivity and Specificity; Short Report; Brazil

BACKGROUND: Bacteria associated with insects can have a substantial impact on the biology and life cycle of their host. The checkerboard DNA-DNA hybridization technique is a semi-quantitative technique that has been previously employed in odontology to detect and quantify a variety of bacterial spec...