Influence of spironolactone on neonatal screening for congenital adrenal hyperplasia

• Published in: Archives of Disease in Childhood - Fetal and Neonatal Edition Vol. 81; no. 3; pp. F179 - F183
• Main Authors: Terai, Itaru, Yamano, Kimiaki, Ichihara, Naoshi, Arai, Junri, Kobayashi, Kunihiko
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health 01.11.1999; BMJ; BMJ Publishing Group LTD; BMJ Group
• Subjects: 17-alpha-Hydroxyprogesterone; 17-alpha-Hydroxyprogesterone - metabolism; 17α-hydroxyprogesterone; Adrenal Hyperplasia, Congenital; Adrenal Hyperplasia, Congenital - blood; Adrenal Hyperplasia, Congenital - diagnosis; Adrenals. Adrenal axis. Renin-angiotensin system (diseases); Biological and medical sciences; Blood; congenital adrenal hyperplasia; Diuretics; Diuretics - metabolism; Endocrinopathies; Enzyme-Linked Immunosorbent Assay; Enzymes; False Positive Reactions; Humans; Immunological methods for diagnosis and exploration; Immunopathology; Infant, Newborn; Laboratories; Liquid chromatography; Male; Medical sciences; Metabolites; Neonatal Screening; Neonatal Screening - methods; Non tumoral diseases. Target tissue resistance. Benign neoplasms; Original; Original article; Other methods; screening; Spironolactone; Spironolactone - metabolism; Japan; Endocrinopathy; Human; Adrenal cortex diseases; False positive; Hyperadrenocorticism; Medical screening; Enzymopathy; Congenital adrenal hyperplasia syndrome; Newborn; Treatment; Spironolactone; Adrenal gland diseases; ELISA assay

AIM To determine if the diuretic spironolactone cross reacts with 17α-hydroxyprogesterone (17OHP) in an enzyme linked immunosorbent assay (ELISA) kit used for the mass screening of congenital adrenal hyperplasia. METHODS Concentrations of 17OHP on a blood filter paper disc were measured using an ELI...