CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells

• Published in: Current gene therapy Vol. 15; no. 5; p. 447
• Main Authors: Bellec, Jessica, Bacchetta, Marc, Losa, Davide, Anegon, Ignacio, Chanson, Marc, Nguyen, Tuan Huy
• Format: Journal Article
• Language: English
• Published: United Arab Emirates 25.08.2015
• Subjects: Cell Line; Cell Movement - genetics; Cell Proliferation - genetics; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; Cystic Fibrosis - genetics; Cystic Fibrosis Transmembrane Conductance Regulator - genetics; Epithelial Cells - metabolism; Gene Expression - genetics; Genetic Therapy - methods; Genetic Vectors - genetics; Genome - genetics; Humans; Interleukin-8 - genetics; Lentivirus - genetics; Respiratory System - metabolism; RNA Interference - physiology; RNA, Small Interfering - genetics

Polarized airway epithelial cell cultures modelling Cystic Fibrosis Transmembrane conductance Regulator (CFTR) defect are crucial for CF and biomedical research. RNA interference has proven its value to generate knockdown models for various pathologies. More recently, genome editing using CRISPR-Cas...