REAP: A two minute cell fractionation method

• Published in: BMC research notes Vol. 3; no. 1; p. 294
• Main Authors: Suzuki, Keiko, Bose, Pinaki, Leong-Quong, Rebecca YY, Fujita, Donald J, Riabowol, Karl
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.11.2010; BioMed Central; BMC
• Subjects: Breast cancer; Cell fractionation; Cold; Fractionation; Life sciences; Medical research; Membranes; Methods; Proteins; Proteolysis; Short Report; Sucrose; Canada

The translocation or shuttling of proteins between the nucleus and cytoplasm (nucleocytoplasmic transport [NCPT]) is often a rapid event following stimulation with growth factors or in response to stress or other experimental manipulations. Commonly used methods to separate nuclei from cytoplasm emp...