Changes in prevalence and load of airway bacteria using quantitative PCR in stable and exacerbated COPD

• Published in: Thorax Vol. 67; no. 12; pp. 1075 - 1080
• Main Authors: Garcha, Davinder S, Thurston, Sarah J, Patel, Anant R C, Mackay, Alexander J, Goldring, James J P, Donaldson, Gavin C, McHugh, Timothy D, Wedzicha, Jadwiga A
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and British Thoracic Society 01.12.2012; BMJ Publishing Group; BMJ Publishing Group LTD
• Subjects: Aged; Analysis of Variance; Bacteria; bacterial load; Batch processing; Biological and medical sciences; Cardiology. Vascular system; Chi-Square Distribution; Chronic obstructive pulmonary disease; Chronic obstructive pulmonary disease, asthma; colonisation; COPD; Dyspnea; exacerbations; Female; Haemophilus influenzae; Haemophilus influenzae - isolation & purification; Humans; Inflammation; Linear Models; London; Male; Medical sciences; Moraxella catarrhalis; Moraxella catarrhalis - isolation & purification; Mortality; Pneumology; Polymerase Chain Reaction - methods; Prevalence; Proteins; Pulmonary Disease, Chronic Obstructive - microbiology; Pulmonary Disease, Chronic Obstructive - physiopathology; quantitative PCR; Respiratory Function Tests; Sputum - microbiology; Staphylococcus infections; Steroids; Streptococcus pneumoniae; Streptococcus pneumoniae - isolation & purification; London; United Kingdom > UK; Lung disease; Load; Prevalence; Respiratory disease; Change; Polymerase chain reaction; Respiratory tract; Chronic; Bronchus disease; Anesthesia; Bacteria; Chronic obstructive pulmonary disease; Circulatory system; Obstructive pulmonary disease; Molecular biology; Cardiology; Quantitative analysis

Background Prevalence and load of airway bacteria in stable and exacerbated chronic obstructive pulmonary disease (COPD) has been previously studied using microbiological culture. Molecular techniques, such as quantitative PCR (qPCR), may be more informative. Methods In this study, 373 sputum sample...