Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites

• Published in: BMC oral health Vol. 14; no. 1; p. 157
• Main Authors: Schaumann, Simone, Staufenbiel, Ingmar, Scherer, Ralph, Schilhabel, Markus, Winkel, Andreas, Stumpp, Sascha Nico, Eberhard, Jörg, Stiesch, Meike
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 17.12.2014; BioMed Central
• Subjects: Actinomycetaceae - classification; Actinomycetaceae - genetics; Aggregatibacter - classification; Aggregatibacter - genetics; Analysis; Bacteria - classification; Bacteria - genetics; Bacteroides - classification; Bacteroides - genetics; Biofilms - classification; Biomedical materials; Dental Deposits - microbiology; Dental Implants - microbiology; Dental Plaque Index; Dentistry; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Bacterial - analysis; Family medical history; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - genetics; Gram-positive bacteria; Gram-Positive Bacteria - classification; Health aspects; High-Throughput Nucleotide Sequencing; Humans; Hybridization; Implant dentures; Materials science; Nucleotide sequencing; Periodontitis - microbiology; Porphyromonas - classification; Porphyromonas - genetics; Prevotella - classification; Prevotella - genetics; Prostheses; RNA, Ribosomal, 16S - analysis; Sequence Analysis, DNA; Streptococcaceae - classification; Streptococcaceae - genetics; Teeth; Germany

To investigate the microbial composition of biofilms at inflamed peri-implant and periodontal tissues in the same subject, using 16S rRNA sequencing. Supra- and submucosal, and supra- and subgingival plaque samples were collected from 7 subjects suffering from diseased peri-implant and periodontal t...