Identification of resident and inflammatory bone marrow derived cells in the sclera by bone marrow and haematopoietic stem cell transplantation

• Published in: British journal of ophthalmology Vol. 91; no. 4; pp. 520 - 526
• Main Authors: Hisatomi, Toshio, Sonoda, Koh-hei, Ishikawa, Fumihiko, Qiao, Hong, Nakazawa, Takahiro, Fukata, Mitsuhiro, Nakamura, Toru, Noda, Kousuke, Miyahara, Shinsuke, Harada, Mine, Kinoshita, Shigeru, Hafezi-Moghadam, Ali, Ishibashi, Tatsuro, Miller, Joan W
• Format: Journal Article
• Language: English
• Published: BMA House, Tavistock Square, London, WC1H 9JR BMJ Publishing Group Ltd 01.04.2007; BMJ; BMJ Publishing Group LTD; BMJ Group
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; antigen presenting cells; Antigens; APC; Autoimmune Diseases - pathology; Biological and medical sciences; Bone marrow; Bone Marrow Cells - ultrastructure; Bone Marrow Transplantation - pathology; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Differentiation; Cell Movement; Cloning; Dendritic cells; Dendritic Cells - pathology; EAU; experimental autoimmune uveitis; Fibroblasts; GFP; green fluorescent protein; haematopoietic stem cells; Hematopoietic Stem Cell Transplantation; HSC; Immunology; Immunophenotyping; Laboratory Science - Extended Report; Macrophages - pathology; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microscopy, Fluorescence; Miscellaneous; Ophthalmology; PBS; phosphate buffered saline; scanning electron microscopy; Sclera - immunology; Sclera - ultrastructure; SEM; Stem cells; Studies; TEM; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transgenic animals; transmission electron microscopy; Uveitis - pathology; California; Human; Resident(student); Hematopoietic cell; Bone marrow; Stem cell transplantation; Resident; Identification; Inflammatory cell; Ophthalmology; Sclera

Aims: To characterise bone marrow derived cells in the sclera under normal and inflammatory conditions, we examined their differentiation after transplantation from two different sources, bone marrow and haematopoietic stem cells (HSC). Methods: Bone marrow and HSC from green fluorescent protein (GF...