Cloning, purification, and functional characterization of Carocin S2, a ribonuclease bacteriocin produced by Pectobacterium carotovorum

• Published in: BMC microbiology Vol. 11; no. 1; p. 99
• Main Authors: Chan, Yung-Chieh, Wu, Jian-Li, Wu, Huang-Pin, Tzeng, Kuo-Ching, Chuang, Duen-Yau
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.05.2011; BioMed Central; BMC
• Subjects: Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Bacteria, Phytopathogenic; Bacteriocins; Bacteriocins - biosynthesis; Bacteriocins - chemistry; Bacteriocins - genetics; Cloning, Molecular; DNA sequencing; DNA Transposable Elements; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli - genetics; Gene Expression; Gene Expression Regulation, Bacterial - radiation effects; Genetic aspects; Genomes; Molecular Sequence Data; Molecular Weight; Mutagenesis, Insertional; Nucleotide sequencing; Open Reading Frames; Pectobacterium carotovorum - genetics; Pectobacterium carotovorum - metabolism; Physiological aspects; Plasmids; Ribonucleases - biosynthesis; Ribonucleases - chemistry; Ribonucleases - genetics; Sequence Analysis, DNA; Transcriptional Activation; Ultraviolet Rays; Taiwan

Most isolates of Pectobacterium carotovorum subsp. carotovorum (Pcc) produce bacteriocins. In this study, we have determined that Pcc strain F-rif-18 has a chromosomal gene encoding the low-molecular-weight bacteriocin, Carocin S2, and that this bacteriocin inhibits the growth of a closely related s...