Enhancer trapping in zebrafish using the Sleeping Beauty transposon

• Published in: BMC genomics Vol. 5; no. 1; p. 62
• Main Authors: Balciunas, Darius, Davidson, Ann E, Sivasubbu, Sridhar, Hermanson, Spencer B, Welle, Zachary, Ekker, Stephen C
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.09.2004; BioMed Central; BMC
• Subjects: Animals; Danio rerio; DNA Transposable Elements; Drosophila; Embryonic Development - genetics; Enhancer Elements, Genetic; Freshwater; Gene Transfer Techniques; Genomics - methods; Germ Cells; Glycoside Hydrolases - genetics; Green Fluorescent Proteins - biosynthesis; In Situ Hybridization; Metazoa; Motor Neurons - metabolism; Pilot Projects; Polymerase Chain Reaction; Promoter Regions, Genetic; Sequence Analysis, DNA - methods; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism

Among functional elements of a metazoan gene, enhancers are particularly difficult to find and annotate. Pioneering experiments in Drosophila have demonstrated the value of enhancer "trapping" using an invertebrate to address this functional genomics problem. We modulated a Sleeping Beauty...