Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction

• Published in: Gut Vol. 53; no. 4; pp. 523 - 529
• Main Authors: Fite, A, Macfarlane, G T, Cummings, J H, Hopkins, M J, Kong, S C, Furrie, E, Macfarlane, S
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and British Society of Gastroenterology 01.04.2004; BMJ; BMJ Publishing Group Ltd; BMJ Publishing Group LTD; Copyright 2004 by Gut
• Subjects: Adult; Age; Age Factors; Aged; Bacteria; Bacteriological Techniques; Base Sequence; Biological and medical sciences; Biopsy; Care and treatment; Colitis, Ulcerative - microbiology; Desulfovibrio - classification; Desulfovibrio - genetics; Desulfovibrio - isolation & purification; desulfovibrios; Desulfuration; DNA, Bacterial - analysis; Epithelial cells; Feces - microbiology; Female; Gastroenterology. Liver. Pancreas. Abdomen; Health aspects; Humans; IBD; Inflammatory bowel disease; intestinal bacteria; Intestinal Microflora; Intestinal Mucosa - microbiology; Male; Medical research; Medical sciences; Metabolism; Metabolites; Methods; Microorganisms; Middle Aged; Molecular Sequence Data; mucosal bacteria; Older people; PCR; Polymerase chain reaction; Polymerase Chain Reaction - methods; Real time; Rectum - microbiology; ribosomal RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA; Sequence Alignment; SRB; Studies; Sulfate-reducing bacteria; sulphate reducing bacteria; ulcerative colitis; United States; United Kingdom > UK; Polymerase chain reaction; Desulfovibrio; Gastroenterology; Bacteria; Identification; Feces; Molecular biology; Real time

Background: Desulfovibrios produce sulphide, which is toxic to colonic epithelial cells. These bacteria have previously been linked to ulcerative colitis. Traditional methods of culturing these organisms are slow, and often unreliable, while molecular approaches are either non-quantitative or lack s...