Applying Pollen DNA Metabarcoding to the Study of Plant–Pollinator Interactions

• Published in: Applications in plant sciences Vol. 5; no. 6
• Main Authors: Bell, Karen L, Fowler, Julie, Burgess, Kevin S, Dobbs, Emily K, Gruenewald, David, Lawley, Brice, Morozumi, Connor, Brosi, Berry J
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01.06.2017; John Wiley & Sons, Inc
• Subjects: Application; APPLICATION ARTICLES; bees; Contamination; Deoxyribonucleic acid; DNA; DNA barcoding; DNA metabarcoding; Florida; Forest management; genes; Identification; ITS; light microscopy; Methods; Nucleotide sequence; palynology; Plant reproduction; Plant sciences; plant–pollinator interactions; Pollen; Pollination; pollination networks; rbcL; Studies; Taxonomy; Florida; United States > US; palynology; ITS; plant–pollinator interactions; rbcL; DNA metabarcoding; pollination networks

Premise of the study: To study pollination networks in a changing environment, we need accurate, high-throughput methods. Previous studies have shown that more highly resolved networks can be constructed by studying pollen loads taken from bees, relative to field observations. DNA metabarcoding pote...