Molecular characterization of a fungal gene paralogue of the penicillin penDE gene of Penicillium chrysogenum

• Published in: BMC microbiology Vol. 9; no. 1; p. 104
• Main Authors: García-Estrada, Carlos, Vaca, Inmaculada, Ullán, Ricardo V, van den Berg, Marco A, Bovenberg, Roel AL, Martín, Juan Francisco
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 26.05.2009; BioMed Central; BMC
• Subjects: Acyltransferases - genetics; Acyltransferases - metabolism; Amino Acid Sequence; DNA, Fungal - genetics; Escherichia coli; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Genes, Fungal; Genetic aspects; Introns; Molecular Sequence Data; Mutagenesis, Site-Directed; Penicillin; Penicillin-Binding Proteins - genetics; Penicillin-Binding Proteins - metabolism; Penicillins - biosynthesis; Penicillium chrysogenum - enzymology; Penicillium chrysogenum - genetics; Peroxisomes - enzymology; Research article; Sequence Alignment; Sequence Analysis, DNA

Penicillium chrysogenum converts isopenicillin N (IPN) into hydrophobic penicillins by means of the peroxisomal IPN acyltransferase (IAT), which is encoded by the penDE gene. In silico analysis of the P. chrysogenum genome revealed the presence of a gene, Pc13g09140, initially described as paralogue...