Novel insights into RNAi off-target effects using C. elegans paralogs

• Published in: BMC genomics Vol. 8; no. 1; p. 106
• Main Authors: Rual, Jean-François, Klitgord, Niels, Achaz, Guillaume
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.04.2007; BioMed Central; BMC
• Subjects: Animals; Base Pair Mismatch - genetics; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans Proteins - physiology; Computational Biology; Genetic aspects; Genetic Techniques - statistics & numerical data; Methodology; Models, Chemical; Ribonuclease III; RNA Interference - drug effects; RNA, Double-Stranded - chemistry; RNA, Double-Stranded - metabolism; RNA, Small Interfering - chemistry; RNA, Small Interfering - genetics; Sensitivity and Specificity; France

In the few years since its discovery, RNAi has turned into a very powerful tool for the study of gene function by allowing post-transcriptional gene silencing. The RNAi mechanism, which is based on the introduction of a double-stranded RNA (dsRNA) trigger whose sequence is similar to that of the tar...