Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity - Ouattara, Bazoumana; Duplessis, Mélissa; Girard, Christiane L (BMC biochemistry, 2013)
Keywords: Analysis; Analytical chemistry; Animal lactation; Animals; Biocatalysis; Biochemistry; Biological products; Biomedical and Life Sciences; Cattle; Chromatography; Chromatography, High Pressure Liquid - standards; Chromatography, Reverse-Phase - standards; Coenzymes; Enzyme Assays - methods; Enzymes; Fermentation; Half-Life; High performance liquid chromatography; Life Sciences; Liquid chromatography; Liver; Liver - enzymology; Measurement; Methodology; Methodology Article; Methods; Methylmalonyl-CoA Mutase - metabolism; Methylmalonyl-CoA Mutase - pharmacokinetics; Methylmalonyl-CoA Mutase - standards; Optimization; Physiological aspects; Protein and enzyme biochemistry; Protein Stability; Proteins; Reproducibility of Results; Studies; Surfactants; Temperature; Thiols; Canada; RP-HPLC; Dairy cow; Succinyl-CoA; Cattle; Liver; Methylmalonyl-CoA mutase