Systematic analysis of mRNA 5' coding sequence incompleteness in Danio rerio: an automated EST-based approach

• Published in: Biology direct Vol. 2; no. 1; p. 34
• Main Authors: Frabetti, Flavia, Casadei, Raffaella, Lenzi, Luca, Canaider, Silvia, Vitale, Lorenza, Facchin, Federica, Carinci, Paolo, Zannotti, Maria, Strippoli, Pierluigi
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27.11.2007; BioMed Central; BMC
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cloning; Cloning, Molecular; Cluster Analysis; Computational Biology; Databases, Factual; DNA, Complementary - genetics; Expressed Sequence Tags; Genetic aspects; Messenger RNA; Molecular Sequence Data; Open Reading Frames - genetics; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Software; Zebra fish; Zebrafish - genetics; Zebrafish Proteins - genetics; United States

All standard methods for cDNA cloning are affected by a potential inability to effectively clone the 5' region of mRNA. The aim of this work was to estimate mRNA open reading frame (ORF) 5' region sequence completeness in the model organism Danio rerio (zebrafish). We implemented a novel a...