PCR colorimetric dot-blot assay and clinical pretest probability for diagnosis of Pulmonary Tuberculosis in Smear-Negative patients

• Published in: BMC public health Vol. 7; no. 1; p. 356
• Main Authors: Scherer, Luciene Cardoso, Sperhacke, Rosa Dea, Jarczewski, Carla, Cafrune, Patrícia I, Minghelli, Simone, Ribeiro, Marta Osório, Mello, Fernanda CQ, Ruffino-Netto, Antonio, Rossetti, Maria LR, Kritski, Afrânio L
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 20.12.2007; BioMed Central; BMC
• Subjects: Adult; Brazil; Care and treatment; Causes of; Colorimetry; Cough; Developing Countries; Diagnosis; DNA, Bacterial - analysis; Health aspects; Humans; Methods; Molecular Probe Techniques; Mycobacterium tuberculosis; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Nucleotide Mapping; Polymerase chain reaction; Polymerase Chain Reaction - methods; Probability; Pulmonary tuberculosis; Sensitivity and Specificity; Sputum - microbiology; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - epidemiology; Tuberculosis, Pulmonary - microbiology; Brazil

Smear-negative pulmonary tuberculosis (SNPTB) accounts for 30% of Pulmonary Tuberculosis (PTB) cases reported annually in developing nations. Polymerase chain reaction (PCR) may provide an alternative for the rapid detection of Mycobacterium tuberculosis (MTB); however little data are available rega...