Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

• Published in: BMC cancer Vol. 6; no. 1; p. 250
• Main Authors: Solmi, Rossella, Ugolini, Giampaolo, Rosati, Giancarlo, Zanotti, Simone, Lauriola, Mattia, Montroni, Isacco, del Governatore, Marco, Caira, Antonello, Taffurelli, Mario, Santini, Donatella, Coppola, Domenico, Guidotti, Lia, Carinci, Paolo, Strippoli, Pierluigi
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 20.10.2006; BioMed Central; BMC
• Subjects: Adult; Aged; Automation; Colonic Neoplasms - blood; Epithelial Cells - chemistry; Female; Gene Expression Profiling - methods; Humans; Keratin-20; Keratins - blood; Male; Membrane Proteins - genetics; Middle Aged; Neoplastic Cells, Circulating - chemistry; Nuclear Proteins - genetics; Nucleocytoplasmic Transport Proteins - genetics; Oligonucleotide Array Sequence Analysis - methods; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - blood; RNA, Neoplasm - blood; Serine Endopeptidases - genetics

The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality...