Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality...
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Published in | BMC cancer Vol. 6; no. 1; p. 250 |
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Main Authors | , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
BioMed Central Ltd
20.10.2006
BioMed Central BMC |
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Abstract | The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions.
In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription-polymerase chain reaction (RT-PCR).
Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify.
The design of new approaches to identify such markers is warranted. |
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AbstractList | The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions.
In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription-polymerase chain reaction (RT-PCR).
Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify.
The design of new approaches to identify such markers is warranted. Background: The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high- throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription - polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. BACKGROUNDThe efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions.METHODSIn this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription-polymerase chain reaction (RT-PCR).RESULTSOur present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify.CONCLUSIONThe design of new approaches to identify such markers is warranted. Abstract Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. |
ArticleNumber | 250 |
Author | Lauriola, Mattia Ugolini, Giampaolo Caira, Antonello Montroni, Isacco Coppola, Domenico Taffurelli, Mario Solmi, Rossella Carinci, Paolo Zanotti, Simone Rosati, Giancarlo Santini, Donatella Guidotti, Lia Strippoli, Pierluigi del Governatore, Marco |
AuthorAffiliation | 3 "H.Lee Moffit" Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA 4 Department of Pathology, University of Bologna, Via Massarenti 9, I-40138 Bologna, Italy 2 Department of Surgical and Anesthesiological Sciences-General Surgery, University of Bologna, Via Massarenti 9, I-40138 Bologna, Italy 1 Department of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, I-40126 Bologna, Italy |
AuthorAffiliation_xml | – name: 4 Department of Pathology, University of Bologna, Via Massarenti 9, I-40138 Bologna, Italy – name: 3 "H.Lee Moffit" Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA – name: 2 Department of Surgical and Anesthesiological Sciences-General Surgery, University of Bologna, Via Massarenti 9, I-40138 Bologna, Italy – name: 1 Department of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, I-40126 Bologna, Italy |
Author_xml | – sequence: 1 givenname: Rossella surname: Solmi fullname: Solmi, Rossella email: solmi@alma.unibo.it organization: Department of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, I-40126 Bologna, Italy. solmi@alma.unibo.it – sequence: 2 givenname: Giampaolo surname: Ugolini fullname: Ugolini, Giampaolo – sequence: 3 givenname: Giancarlo surname: Rosati fullname: Rosati, Giancarlo – sequence: 4 givenname: Simone surname: Zanotti fullname: Zanotti, Simone – sequence: 5 givenname: Mattia surname: Lauriola fullname: Lauriola, Mattia – sequence: 6 givenname: Isacco surname: Montroni fullname: Montroni, Isacco – sequence: 7 givenname: Marco surname: del Governatore fullname: del Governatore, Marco – sequence: 8 givenname: Antonello surname: Caira fullname: Caira, Antonello – sequence: 9 givenname: Mario surname: Taffurelli fullname: Taffurelli, Mario – sequence: 10 givenname: Donatella surname: Santini fullname: Santini, Donatella – sequence: 11 givenname: Domenico surname: Coppola fullname: Coppola, Domenico – sequence: 12 givenname: Lia surname: Guidotti fullname: Guidotti, Lia – sequence: 13 givenname: Paolo surname: Carinci fullname: Carinci, Paolo – sequence: 14 givenname: Pierluigi surname: Strippoli fullname: Strippoli, Pierluigi |
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Snippet | The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early... Background: The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation... BACKGROUNDThe efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at... BACKGROUND: The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation... Abstract Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the... |
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SubjectTerms | Adult Aged Automation Colonic Neoplasms - blood Epithelial Cells - chemistry Female Gene Expression Profiling - methods Humans Keratin-20 Keratins - blood Male Membrane Proteins - genetics Middle Aged Neoplastic Cells, Circulating - chemistry Nuclear Proteins - genetics Nucleocytoplasmic Transport Proteins - genetics Oligonucleotide Array Sequence Analysis - methods Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - blood RNA, Neoplasm - blood Serine Endopeptidases - genetics |
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Title | Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
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