Regulation by epidermal growth factor of prostaglandin production and cyclooxygenase activity in sensitized rat endometrial stromal cells in vitro
The effect of epidermal growth factor on prostaglandin production and cyclooxygenase activity in endometrial stromal cells isolated from the uteri of ovariectomized rats sensitized for the decidual cell reaction was examined. Treatment with epidermal growth factor (40 ng ml −1 ) for 24 h caused appr...
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Published in | Journal of reproduction & fertility Vol. 104; no. 1; pp. 57 - 62 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Society for Reproduction and Fertility
01.05.1995
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Subjects | |
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Abstract | The effect of epidermal growth factor on prostaglandin production and cyclooxygenase activity in endometrial stromal cells
isolated from the uteri of ovariectomized rats sensitized for the decidual cell reaction was examined. Treatment with epidermal
growth factor (40 ng ml −1 ) for 24 h caused approximately 6.9-, 3.4-, and 4.1-fold increases in prostaglandin E 2 , prostaglandin F 2α and 13,14-dihydro-15-keto-prostaglandin F 2α accumulation in the media of cultured cells. The increase in prostaglandin E 2 accumulation induced by epidermal growth factor was inhibited by α-amanitin (2 μg ml −1 ), cycloheximide (0.5 μg ml −1 ) and dexamethasone (5 μmol l −1 ). Epidermal growth factor increased cyclooxygenase activity in the stromal cells in a time-dependent fashion and this increase
in activity was also inhibited by α-amanitin, cycloheximide and dexamethasone. These results provide evidence that epidermal
growth factor stimulates prostaglandin production in rat endometrial stromal cells from uteri sensitized for the decidual
cell reaction through a mechanism that involves an increase in cyclooxygenase activity. |
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AbstractList | The effect of epidermal growth factor on prostaglandin production and cyclooxygenase activity in endometrial stromal cells isolated from the uteri of ovariectomized rats sensitized for the decidual cell reaction was examined. Treatment with epidermal growth factor (40 ng ml-1) for 24 h caused approximately 6.9., 3.4-, and 4.1-fold increases in prostaglandin E2, prostaglandin F2 alpha and 13,14-dihydro-15-keto- prostaglandin F2 alpha accumulation in the media of cultured cells. The increase in prostaglandin E2 accumulation induced by epidermal growth factor was inhibited by alpha-amanitin (2 micrograms ml-1), cycloheximide (0.5 micrograms ml-1) and dexamethasone (5 mumol l-1). Epidermal growth factor increased cyclooxygenase activity in the stromal cells in a time-dependent fashion and this increase in activity was also inhibited by alpha-amanitin, cycloheximide and dexamethasone. These results provide evidence that epidermal growth factor stimulates prostaglandin production in rat endometrial stromal cells from uteri sensitized for the decidual cell reaction through a mechanism that involves an increase in cyclooxygenase activity. The effect of epidermal growth factor on prostaglandin production and cyclooxygenase activity in endometrial stromal cells isolated from the uteri of ovariectomized rats sensitized for the decidual cell reaction was examined. Treatment with epidermal growth factor (40 ng ml −1 ) for 24 h caused approximately 6.9-, 3.4-, and 4.1-fold increases in prostaglandin E 2 , prostaglandin F 2α and 13,14-dihydro-15-keto-prostaglandin F 2α accumulation in the media of cultured cells. The increase in prostaglandin E 2 accumulation induced by epidermal growth factor was inhibited by α-amanitin (2 μg ml −1 ), cycloheximide (0.5 μg ml −1 ) and dexamethasone (5 μmol l −1 ). Epidermal growth factor increased cyclooxygenase activity in the stromal cells in a time-dependent fashion and this increase in activity was also inhibited by α-amanitin, cycloheximide and dexamethasone. These results provide evidence that epidermal growth factor stimulates prostaglandin production in rat endometrial stromal cells from uteri sensitized for the decidual cell reaction through a mechanism that involves an increase in cyclooxygenase activity. |
Author | B. M. Bany T. G. Kennedy |
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Snippet | The effect of epidermal growth factor on prostaglandin production and cyclooxygenase activity in endometrial stromal cells
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SubjectTerms | Amanitins - pharmacology Animals Cells, Cultured Cycloheximide - pharmacology Dexamethasone - pharmacology Dinoprost - analogs & derivatives Dinoprost - metabolism Dinoprostone - metabolism Endometrium - metabolism Epidermal Growth Factor - pharmacology Female Prostaglandin-Endoperoxide Synthases - metabolism Prostaglandins - biosynthesis Rats Rats, Sprague-Dawley Stimulation, Chemical Time Factors Transcription, Genetic - drug effects |
Title | Regulation by epidermal growth factor of prostaglandin production and cyclooxygenase activity in sensitized rat endometrial stromal cells in vitro |
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