Clinical utility of a nested nucleic acid amplification format in comparison to viral culture for the diagnosis of mucosal herpes simplex infection in a genitourinary medicine setting

• Published in: BMC infectious diseases Vol. 1; no. 1; p. 11
• Main Authors: Coyle, P V, O'Neill, H J, McCaughey, C, Wyatt, D E, McBride, M O
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 21.08.2001; BioMed Central; BMC
• Subjects: Animals; Cell Culture Techniques; Cell Line; Haplorhini; Herpes Simplex - diagnosis; Herpes Simplex - virology; Humans; Mucous Membrane - virology; Polymerase Chain Reaction - methods; Simplexvirus - isolation & purification; Simplexvirus - physiology; Urogenital System

Nested nucleic acid amplification tests are often thought too sensitive or prone to generating false positive results for routine use. The current study investigated the specificity and clinical utility of a routine multiplex nested assay for mucosal herpetic infections. Ninety patients, categorised...