The effect of epidermal growth factor and IGF-I infusion on hepatic and renal expression of the IGF-system in adult female rats

• Published in: Journal of endocrinology Vol. 165; no. 1; pp. 115 - 122
• Main Authors: van Neck, JW, Berghout, EM, Vinter-Jensen, L, Groffen, CA, Cingel, V, Dits, NF, Drop, SL, Flyvbjerg, A
• Format: Journal Article
• Language: English
• Published: Colchester BioScientifica 01.04.2000; Portland Press
• Subjects: Animals; Apud cells. Peptide and protein hormones. Growth factors; ARN MENSAJERO; ARN MESSAGER; Biological and medical sciences; BLOOD SERUM; BODY WEIGHT; Body Weight - drug effects; EPIDERMAL GROWTH FACTOR; Epidermal Growth Factor - pharmacology; EXPRESION GENICA; EXPRESSION DES GENES; FACTEUR DE CROISSANCE EPIDERMIQUE; FACTEUR DE CROISSANCE IGF; FACTOR DE CRECIMIENTO EPIDERMICO; FACTOR DE CRECIMIENTO IGF; Female; FOIE; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; HIGADO; INFUSION; INSULIN-LIKE GROWTH FACTOR; Insulin-Like Growth Factor Binding Proteins - blood; Insulin-Like Growth Factor Binding Proteins - drug effects; Insulin-Like Growth Factor I - pharmacology; Kidney - drug effects; Kidney - metabolism; KIDNEYS; LIVER; Liver - drug effects; Liver - metabolism; MESSENGER RNA; PESO CORPORAL; POIDS CORPOREL; RAT; RATA; RATS; Rats, Wistar; RECEPTORS; REIN; RINONES; RNA, Messenger - drug effects; RNA, Messenger - metabolism; SERUM SANGUIN; SUERO SANGUINEO; Vertebrates: endocrinology; Rat; Digestive system; Liver; Body weight; Rodentia; Gene expression; Insulin like growth factor 1; Kidney; Insulin like growth factor binding protein; Vertebrata; Mammalia; Epidermal growth factor; Urinary system
• ISSN: 0022-0795; 1479-6805
• DOI: 10.1677/joe.0.1650115

Systemic administration of epidermal growth factor (EGF) in neonatal rats results in reduced body weight gain and decreased circulating levels of IGF-I, suggesting its involvement in EGF-induced growth retardation. We investigated the effect of EGF and/or IGF-I administration for 7 days on circulating IGF-I and IGFBP levels and hepatic and renal IGF-system mRNA expression profiles in adult female rats. EGF administration (30 microg/rat/day) did not influence body weight, liver or kidney weight. In contrast, IGF-I (400 microg/rat/day) and EGF/IGF-I administration increased both body weight and kidney weight. Also, serum IGF-I and the 30 kDa IGFBPs (IGFBP-1 and -2) were significantly increased in these groups. Serum IGFBP-3 levels increased in the IGF-I group along with increased hepatic IGFBP-1 and -3 mRNA levels. In contrast, in the EGF administration group serum IGFBP-3 levels were significantly decreased; however, the mRNA levels remained unchanged. In the EGF/IGF-I administration group, serum IGF-I and IGFBP-3 levels were significantly lowered when compared with the IGF-I administration group. This was in contrast to the effect on kidney weight increase that was identical for the IGF-I and EGF/IGF-I groups. The decrease in serum IGFBP-3 was not reflected at the hepatic IGFBP-3 mRNA level. IGFBP-3 expression might be regulated at a post-transcriptional level although EGF induced IGFBP-3 proteolysis could not be demonstrated in vitro. We conclude that EGF administration reduced serum IGFBP-3 whereas IGF-I administration increased the level of IGFBP-3 and IGF-I and resulted in an increased body and kidney weight in adult female rats.