Using Herbarium-Derived DNAs to Assemble a Large-Scale DNA Barcode Library for the Vascular Plants of Canada

• Published in: Applications in plant sciences Vol. 5; no. 12
• Main Authors: Kuzmina, Maria L, Braukmann, Thomas W. A, Fazekas, Aron J, Graham, Sean W, Dewaard, Stephanie L, Rodrigues, Anuar, Bennett, Bruce A, Dickinson, Timothy A, Saarela, Jeffery M, Catling, Paul M, Newmaster, Steven G, Percy, Diana M, Fenneman, Erin, Lauron-Moreau, Aurélien, Ford, Bruce, Gillespie, Lynn, Subramanyam, Ragupathy, Whitton, Jeannette, Jennings, Linda, Metsger, Deborah, Warne, Connor P, Brown, Allison, Sears, Elizabeth, Dewaard, Jeremy R, Zakharov, Evgeny V, Hebert, Paul D. N
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01.12.2017; John Wiley & Sons, Inc
• Subjects: Age; Application; APPLICATION ARTICLE; Asteraceae; Boraginaceae; Canada; Canadian vascular flora; Cost reduction; Deoxyribonucleic acid; DNA; DNA barcode reference library; DNA barcoding; flora; Flowers & plants; genetic databases; herbaria; herbarium; ITS2; Library collections; matK; metagenomics; Nonnative species; Nucleotide sequence; Plant sciences; Plants; Preservation; rbcL; Taxonomy; vascular plants; British Columbia Canada; Canada; United States > US; Canadian vascular flora; ITS2; matK; herbarium; rbcL; DNA barcode reference library

Premise of the study: Constructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large-scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora...