Crystallization and preliminary diffraction studies of malate dehydrogenase from Streptomyces aureofaciens

• Published in: Protein and peptide letters Vol. 13; no. 2; p. 207
• Main Authors: Mikulásová, Darina, Tomásková, Natasa, Maderová, Jana, Kollárová, Marta
• Format: Journal Article
• Language: English
• Published: Netherlands 01.02.2006
• Subjects: Crystallization; Crystallography, X-Ray; Malate Dehydrogenase - chemistry; Malate Dehydrogenase - genetics; Malate Dehydrogenase - isolation & purification; Malate Dehydrogenase - metabolism; NADP - chemistry; NADP - metabolism; Streptomyces aureofaciens - enzymology; Streptomyces aureofaciens - genetics

Purified malate dehydrogenase (MDH) of Streptomyces aureofaciens was crystallized either in the absence or in the presence of NADH or NADPH coenzymes by hanging-drop vapour-diffusion method. An X-ray study has shown, that MDH crystals belong to space group C222(1) with unit-cell parameters a = 53.2...