Use of V(D)J recombination excision circles to identify T- and B-cell defects and to monitor the treatment in primary and acquired immunodeficiencies

• Published in: Journal of translational medicine Vol. 11; no. 1; p. 119
• Main Authors: Serana, Federico, Chiarini, Marco, Zanotti, Cinzia, Sottini, Alessandra, Bertoli, Diego, Bosio, Andrea, Caimi, Luigi, Imberti, Luisa
• Format: Journal Article
• Language: English
• Published: England BioMed Central 09.05.2013; BioMed Central Ltd
• Subjects: Adult; Anti-Retroviral Agents - therapeutic use; B-Lymphocytes - immunology; Bone marrow; Child; Child, Preschool; DNA, Circular - genetics; Flow Cytometry; HIV Infections - drug therapy; HIV Infections - immunology; Human immunodeficiency virus; Humans; Immune system; Immunologic Deficiency Syndromes - genetics; Immunology; Infant; Infant, Newborn; Kinases; Neonatal Screening; Proteins; Real-Time Polymerase Chain Reaction - methods; Receptors, Antigen, T-Cell - metabolism; Recombination, Genetic; Review; Stem Cell Transplantation; Stem Cells - cytology; T cell receptors; T-Lymphocytes - immunology; V(D)J Recombination

T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles (KRECs) are circular DNA segments generated in T and B cells during their maturation in the thymus and bone marrow. These circularized DNA elements persist in the cells, are unable to replicate, and are dilute...