Effects of omega-3 and -6 polyunsaturated fatty acids on ovine follicular cell steroidogenesis, embryo development and molecular markers of fatty acid metabolism

• Published in: Reproduction (Cambridge, England) Vol. 141; no. 1; pp. 105 - 118
• Main Authors: Hughes, Jaime, Kwong, Wing Yee, Li, Dongfang, Salter, Andrew M, Lea, Richard G, Sinclair, Kevin D
• Format: Journal Article
• Language: English
• Published: England BioScientifica 01.01.2011; BioScientifica Ltd; Society for Reproduction and Fertility
• Subjects: Albumin; Animals; Antioxidants; Blastocyst - drug effects; Blastocyst - metabolism; blastocysts; Cell culture; Cells, Cultured; Diets; Embryo Culture Techniques; Embryos; Enzymes; Fatty acids; Fatty Acids - metabolism; Fatty Acids, Omega-3 - pharmacology; Fatty Acids, Omega-6 - pharmacology; Female; Follicular fluid; Follicular Fluid - metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Granulosa Cells - drug effects; Granulosa Cells - metabolism; Guanylate cyclase; Lipids; Lipoproteins; Lipoproteins (high density); Lipoproteins, HDL - metabolism; Lipoproteins, LDL - metabolism; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; Phosphoproteins - genetics; Phosphoproteins - metabolism; Polyunsaturated fatty acids; Progesterone; Progesterone - biosynthesis; RNA, Messenger - metabolism; Serum Albumin - metabolism; Sheep; Steroidogenesis; Superoxide dismutase; Theca; Theca Cells - drug effects; Theca Cells - metabolism; Time Factors; Transcription

We previously reported increased follicular fluid progesterone (P4) concentrations in ewes fed an n-3 compared to an n-6 polyunsaturated fatty acid (PUFA)-enriched diet, but detected no differential effect of n-3 and n-6 PUFA-enriched high-density lipoproteins (HDL) on granulosa cell (GC) steroidoge...