Distribution of O-acetylated sialomucin in the normal and diseased gastrointestinal tract shown by a new monoclonal antibody

• Published in: Journal of clinical pathology Vol. 46; no. 4; pp. 323 - 329
• Main Authors: Milton, J D, Eccleston, D, Parker, N, Raouf, A, Cubbin, C, Hoffman, J, Hart, C A, Rhodes, J M
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.04.1993; BMJ; BMJ Publishing Group LTD
• Subjects: Animals; Antibodies, Monoclonal - metabolism; Antibody Specificity; Biological and medical sciences; Colonic Neoplasms - chemistry; Digestive System - chemistry; Enzyme-Linked Immunosorbent Assay; Gastric Mucosa - pathology; Gastroenterology. Liver. Pancreas. Abdomen; Gastrointestinal Diseases - metabolism; Humans; Immunoenzyme Techniques; Medical sciences; Metaplasia - metabolism; Mice; Mice, Inbred BALB C; Mucins - analysis; Other diseases. Semiology; Sialomucins; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Human; Pathology; Stomach; Pathophysiology; Gut; Digestive diseases; Acetylation; Monoclonal antibody

AIMS--To produce and characterise a monoclonal antibody specific for O-acetylated sialomucin and to assess its use in immunohistochemistry on a panel of normal and diseased intestinal tissue samples. METHODS--Mouse monoclonal antibodies were developed following immunisation with highly purified huma...