Pro-inflammatory effects of e-cigarette vapour condensate on human alveolar macrophages

• Published in: Thorax Vol. 73; no. 12; pp. 1161 - 1169
• Main Authors: Scott, Aaron, Lugg, Sebastian T, Aldridge, Kerrie, Lewis, Keir E, Bowden, Allen, Mahida, Rahul Y, Grudzinska, Frances Susanna, Dosanjh, Davinder, Parekh, Dhruv, Foronjy, Robert, Sapey, Elizabeth, Naidu, Babu, Thickett, David R
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group LTD 01.12.2018; BMJ Publishing Group
• Series: Original article
• Subjects: Acetylcysteine - pharmacology; Antioxidants - pharmacology; Apoptosis; Apoptosis - drug effects; Cell Survival - drug effects; Chemokine CCL2 - metabolism; Chromatography; Chronic obstructive pulmonary disease; Complex Mixtures - adverse effects; Cytotoxicity; Electronic cigarettes; Electronic Nicotine Delivery Systems; Gases - adverse effects; Health hazards; Humans; Inflammation; Inflammation - etiology; Inflammation - metabolism; Interleukin-6 - metabolism; Interleukin-8 - metabolism; Macrophages, Alveolar - pathology; Macrophages, Alveolar - physiology; Matrix Metalloproteinase 9 - metabolism; Methods; Necrosis - etiology; Nicotine; Nicotine - adverse effects; Oxidative stress; Phagocytosis - drug effects; Phosphoinositide-3 Kinase Inhibitors; Physiology; Protein Kinase Inhibitors - pharmacology; Reactive Oxygen Species - metabolism; Research parks; Smoking; THP-1 Cells; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Vaping - adverse effects; United Kingdom > UK; United States > US; macrophage biology; oxidative stress; respiratory infection

ObjectiveVaping may increase the cytotoxic effects of e-cigarette liquid (ECL). We compared the effect of unvaped ECL to e-cigarette vapour condensate (ECVC) on alveolar macrophage (AM) function.MethodsAMs were treated with ECVC and nicotine-free ECVC (nfECVC). AM viability, apoptosis, necrosis, cyt...