Culture media affect follicle survival and oocyte maturation in preantral mouse follicle cultures

• Published in: Journal of mammalian ova research Vol. 27; no. 1
• Main Authors: Nonowaki, S., Prefectural Univ. of Hiroshima (Japan), Takahashi, K, Horiuchi, T
• Format: Journal Article
• Language: English
• Published: 01.04.2010
• Subjects: CELL CULTURE; CULTIVO DE CELULAS; CULTURE DE CELLULE; CULTURE MEDIA; FOLICULOS OVARICOS; FOLLICULE OVARIEN; http://www.fao.org/aos/agrovoc#c_10204; http://www.fao.org/aos/agrovoc#c_32465; http://www.fao.org/aos/agrovoc#c_4655; http://www.fao.org/aos/agrovoc#c_4795; http://www.fao.org/aos/agrovoc#c_7178; http://www.fao.org/aos/agrovoc#c_7538; MADURACION; MATURATION; MEDIO DE CULTIVO; MICE; MILIEU DE CULTURE; OVARIAN FOLLICLES; RATON; SOURIS; SUPERVIVENCIA; SURVIE; SURVIVAL
• ISSN: 1341-7738
• DOI: 10.1274/jmor.27.35

To investigate the effect of media on mouse follicular development and oocyte maturation in vitro, we compared eight culture media. Early preantral follicles were mechanically dissected from the ovaries of 14-day-old mice and cultured for 10 days. The tested media were: alpha-MEM, D-MEM/F-12, D-MEM with high glucose (4.5 g/L) (D-MEM[H]) or low glucose (1 g/L) (D-MEM[L]), Waymouth, M199, IMDM, and RPMI1640. All of the media were supplemented with 5% FBS, ITS, 100 mlU/ml rhFSH, 1 mlU/ml rhLH and, 0.5% gentamicin. Compared to the other media (P 0.05), a higher percentage of follicles survived and antral-like cavity formation was seen after 10 days of culture when the follicles were cultured in alpha-MEM, Waymouth, D-MEM/F-12, or D-MEM(L). Among the four media with higher follicle survival, the oocyte diameter on day 10 of culture was largest in alpha-MEM and smallest in Waymouth. The highest percentages of MII oocytes were obtained when follicles were cultured in alpha-MEM, D-MEM(L), and D-MEM/F-12. In contrast, in Waymouth, no MII oocytes were obtained. The media used for mouse preantral follicle growth in vitro affected follicle survival and oocyte maturation. Of the media that we tested, alpha-MEM, D-MEM(L), and D-MEM/F-12 were superior for mouse preantral follicle cultures.