Organoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles

• Published in: Gut Vol. 69; no. 12; pp. 2165 - 2179
• Main Authors: Yan, Helen H N, Siu, Hoi Cheong, Ho, Siu Lun, Yue, Sarah S K, Gao, Yang, Tsui, Wai Yin, Chan, Dessy, Chan, April S, Wong, Jason W H, Man, Alice H Y, Lee, Bernard C H, Chan, Annie S Y, Chan, Anthony K W, Hui, Ho Sang, Cheung, Arthur K L, Law, Wai Lun, Lo, Oswens S H, Yuen, Siu Tsan, Clevers, Hans, Leung, Suet Yi
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd and British Society of Gastroenterology 01.12.2020; BMJ Publishing Group LTD
• Subjects: Adenomatous polyposis coli; Adenomatous Polyposis Coli Protein - genetics; Age; Biobanks; Bone Morphogenetic Protein 2 - genetics; Bone Morphogenetic Protein Receptors, Type I - genetics; Cancer; Cell Adhesion Molecules - genetics; Cell culture; Colon; colon carcinogenesis; Colorectal cancer; colorectal cancer genes; Colorectal carcinoma; Colorectal Neoplasms - genetics; CRISPR; CRISPR-Cas Systems; Early-onset colon cancer; Gene expression; Gene Expression Profiling; Gene Fusion; Genetic engineering; Genetic Profile; Genomes; Humans; Leukemia; Lymphatic system; Models, Genetic; Mutants; Mutation; organoid models; Organoids; Organoids - pathology; Patients; Phenotypes; Proofreading; R-spondin fusion; Receptor Protein-Tyrosine Kinases - genetics; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - genetics; RNF43; serrated neoplasia; Smad4 protein; Smad4 Protein - genetics; Thrombospondins - genetics; Tissue Banks; Transcription; Tumors; Ubiquitin-Protein Ligases - genetics; Up-Regulation; Whole Exome Sequencing; Wnt protein; RNF43; Early-onset colon cancer; colon carcinogenesis; colorectal cancer; organoid models; R-spondin fusion; serrated neoplasia; colorectal cancer genes; gene expression

ObjectiveSporadic early-onset colorectal cancer (EOCRC) has bad prognosis, yet is poorly represented by cell line models. We examine the key mutational and transcriptomic alterations in an organoid biobank enriched in EOCRCs.DesignWe established paired cancer (n=32) and normal organoids (n=18) from...