Enhanced Vitamin K (Menaquinone-7) Production by Bacillus subtilis natto in Biofilm Reactors by Optimization of Glucose-based Medium
Benefits of vitamin K have been reported by many studies recently, due to its ability to reduce the risk of cardiovascular diseases and its potential benefits against osteoporosis. Specifically, menaquinone-7 (MK-7), being the most potent form of vitamin K, has definitely received most of the attent...
Saved in:
Published in | Current pharmaceutical biotechnology Vol. 19; no. 11; p. 917 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.01.2018
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Benefits of vitamin K have been reported by many studies recently, due to its ability to reduce the risk of cardiovascular diseases and its potential benefits against osteoporosis. Specifically, menaquinone-7 (MK-7), being the most potent form of vitamin K, has definitely received most of the attention. Currently, solid or static liquid fermentation strategies are utilized for industrial production of MK-7 by Bacillus strains. However, these strategies face fundamental operational and scale-up issues as well as intense pellicle and biofilm formations which is problematic in static liquid fermentation, due to heat and mass transfer inefficiencies they create.
The purpose of this study was to demonstrate that biofilm reactors will overcome the issues associated with suspended cell reactors when using Bacillus strains to produce MK-7. The expectation is that the use of biofilm reactors will result in a significant increase in the production of MK-7.
Vitamin K production by Bacillus subtilis natto when grown in a biofilm reactor was evaluated at various concentrations of the three major nutrients, glucose, yeast extract and casein. The data was analyzed using response surface methodology (RSM).
The maximum concentration of MK-7 in the biofilm reactors was 20.5±0.5 mg/L, which was a 344 % increase when compared to the amount produced in suspended-cell reactors containing the same optimum media composition.
These results demonstrate the potential of utilizing biofilm reactors for MK-7 production on an industrial scale. |
---|---|
AbstractList | Benefits of vitamin K have been reported by many studies recently, due to its ability to reduce the risk of cardiovascular diseases and its potential benefits against osteoporosis. Specifically, menaquinone-7 (MK-7), being the most potent form of vitamin K, has definitely received most of the attention. Currently, solid or static liquid fermentation strategies are utilized for industrial production of MK-7 by Bacillus strains. However, these strategies face fundamental operational and scale-up issues as well as intense pellicle and biofilm formations which is problematic in static liquid fermentation, due to heat and mass transfer inefficiencies they create.
The purpose of this study was to demonstrate that biofilm reactors will overcome the issues associated with suspended cell reactors when using Bacillus strains to produce MK-7. The expectation is that the use of biofilm reactors will result in a significant increase in the production of MK-7.
Vitamin K production by Bacillus subtilis natto when grown in a biofilm reactor was evaluated at various concentrations of the three major nutrients, glucose, yeast extract and casein. The data was analyzed using response surface methodology (RSM).
The maximum concentration of MK-7 in the biofilm reactors was 20.5±0.5 mg/L, which was a 344 % increase when compared to the amount produced in suspended-cell reactors containing the same optimum media composition.
These results demonstrate the potential of utilizing biofilm reactors for MK-7 production on an industrial scale. |
Author | Berenjian, Aydin Mahdinia, Ehsan Demirci, Ali |
Author_xml | – sequence: 1 givenname: Ehsan surname: Mahdinia fullname: Mahdinia, Ehsan organization: Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, United States – sequence: 2 givenname: Ali surname: Demirci fullname: Demirci, Ali organization: Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, United States – sequence: 3 givenname: Aydin surname: Berenjian fullname: Berenjian, Aydin organization: Faculty of Science and Engineering, The University of Waikato, Hamilton, 3240, New Zealand |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30474527$$D View this record in MEDLINE/PubMed |
BookMark | eNo1kDtPwzAYRS0Eog_4C8hsMAT8OXZsj7QqBdGqCAFrZceOMEqcEjtDmfnhtDymu5x7j3RH6DC0wSF0DuSKgmDXkEtFCRBKiqIACUALoIQROEBDkCLPWA7FAI1ifCc7SEp6jAY5YYJxKoboaxbedCidxa8-6cYH_IAvli7oj97vTZm4xI9da_sy-TZgs8UTXfq67iOOvUm-9hEHnVKLd9WJbytfN_jJ6TK1Xdzjq03yjf_UP_W2wvO6L9voMqPjTrp01vfNCTqqdB3d6V-O0cvt7Hl6ly1W8_vpzSIzTEDKuCPcKKaMAdCOghVFRTSvlHBCGS2VVYwLZzkhgjNibaVlUXHDwXAhraJjdPa7u-lN4-x60_lGd9v1_x30G3jBZP4 |
CitedBy_id | crossref_primary_10_1002_jctb_7053 crossref_primary_10_1016_j_biotechadv_2019_107453 crossref_primary_10_1007_s00449_022_02752_6 crossref_primary_10_1016_j_bcab_2020_101662 crossref_primary_10_1016_j_fsi_2023_108567 crossref_primary_10_1007_s00253_019_10044_5 crossref_primary_10_1016_j_fuel_2022_125053 crossref_primary_10_3390_pr11092593 crossref_primary_10_1007_s00253_020_10998_x crossref_primary_10_1111_jam_14904 crossref_primary_10_1039_D3NP00065F crossref_primary_10_1016_j_synbio_2021_07_002 crossref_primary_10_1007_s00253_020_10470_w crossref_primary_10_1007_s00449_024_03040_1 crossref_primary_10_1556_066_2019_48_4_1 crossref_primary_10_3390_microorganisms10030554 crossref_primary_10_1016_j_bcab_2018_11_022 crossref_primary_10_1016_j_bcab_2022_102548 crossref_primary_10_1016_j_carbpol_2020_115955 crossref_primary_10_1007_s00253_020_10409_1 crossref_primary_10_1002_jctb_7208 crossref_primary_10_1007_s00253_019_09913_w crossref_primary_10_3390_nano13121825 |
ContentType | Journal Article |
Copyright | Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net. |
Copyright_xml | – notice: Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net. |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.2174/1389201020666181126120401 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Pharmacy, Therapeutics, & Pharmacology |
EISSN | 1873-4316 |
ExternalDocumentID | 30474527 |
Genre | Journal Article |
GroupedDBID | --- .5. 0R~ 29F 36B 4.4 53G 5GY AAEGP ABEEF ABJNI ACGFS ACITR ACIWK ACPRK AENEX AFRAH AFUQM AGJNZ ALMA_UNASSIGNED_HOLDINGS ANTIV C1A CGR CS3 CUY CVF DU5 EBS ECM EIF EJD F5P GH2 HZ~ IPNFZ KCGFV NPM O9- P2P RIG |
ID | FETCH-LOGICAL-b471t-5e05b949bb11ae21d76f0a5f97e79ba89d9457ed5007540ddfa86f5b51b578d92 |
IngestDate | Thu May 23 23:35:13 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 11 |
Keywords | glucose medium Bacillus subtilis menaquinone-7 RSM optimization biofilm reactor MK-7 vitamin K |
Language | English |
License | Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net. |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-b471t-5e05b949bb11ae21d76f0a5f97e79ba89d9457ed5007540ddfa86f5b51b578d92 |
PMID | 30474527 |
ParticipantIDs | pubmed_primary_30474527 |
PublicationCentury | 2000 |
PublicationDate | 2018-01-01 |
PublicationDateYYYYMMDD | 2018-01-01 |
PublicationDate_xml | – month: 01 year: 2018 text: 2018-01-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Netherlands |
PublicationPlace_xml | – name: Netherlands |
PublicationTitle | Current pharmaceutical biotechnology |
PublicationTitleAlternate | Curr Pharm Biotechnol |
PublicationYear | 2018 |
SSID | ssj0020882 |
Score | 2.360731 |
Snippet | Benefits of vitamin K have been reported by many studies recently, due to its ability to reduce the risk of cardiovascular diseases and its potential benefits... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 917 |
SubjectTerms | Bacillus subtilis - metabolism Biofilms - growth & development Bioreactors - microbiology Biotechnology - methods Fermentation Glucose - metabolism Vitamin K 2 - analogs & derivatives Vitamin K 2 - isolation & purification Vitamin K 2 - metabolism |
Title | Enhanced Vitamin K (Menaquinone-7) Production by Bacillus subtilis natto in Biofilm Reactors by Optimization of Glucose-based Medium |
URI | https://www.ncbi.nlm.nih.gov/pubmed/30474527 |
Volume | 19 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLY6kKa9IO53ZCQ0gTpDnCV18rhBYWLq2EOH9jbZsaN6arJC04fyzM_hR3JO7KxJYeLyEkVxHCU5n46_czUhLwIhMhNIwXimBMNAEVOBCpjWOg-NNMBh0aE_OhocnEQfT-PTXu9HK2tpUanX2bff1pX8j1ThGsgVq2T_QbKXD4ULcA7yhSNIGI5_JeNhOXEB_M9g4Re27B8iYRyZUn5ZWLDrDUNLH4sBtGsSi2RzX2Z2Ol3M-_OFquzUzvulrICBwvR9ixt4F5hZ73bhgds_gU4pfLEmMssPLsed4fKn6ziPb-Zw3m1jOpt0nOXKXlS_ePFHcgJLp0vXHU5a6UHvTGG_ZtZV4NhLlwGWJp5b57LdW2rfNNz7LHjS8lkYp2cTscuwCr-jiNM24HhLraauvnNd3aM5hZ4HIF0Y1MfO9ANgLBzbooFy4u05ILlZUeMAA41RHIo_j6514m6GNsiGSFCnHqFnyBv3aKpskuf-rd5c-U5bZLN5zpolUzOa8U1yw5sidM_h6hbpmfI22T52clvu0PGqNG--Q7fp8arL-fIO-d6Aj3rw0UP6sgO9V3QFPKqWtAEebYBHa-BRmOqBRxvg4e1t4NGLnHaARx3w7pKT98Px2wPmt_RgClhQxWITxCqNUqU4lybkWgzyQMZ5KoxIlUxSnUaxMDpGKhsFoDBkMshjFXMFS4tOw3vkGn7DA0K1yXdNoGsWG4UZVwKMnQT4qBaaC64fkvvu757NXN-Ws-a_P7py5DHZWsH1Cbmeg6IwT4F1VupZLe6fcSGCyA |
link.rule.ids | 783 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Enhanced+Vitamin+K+%28Menaquinone-7%29+Production+by+Bacillus+subtilis+natto+in+Biofilm+Reactors+by+Optimization+of+Glucose-based+Medium&rft.jtitle=Current+pharmaceutical+biotechnology&rft.au=Mahdinia%2C+Ehsan&rft.au=Demirci%2C+Ali&rft.au=Berenjian%2C+Aydin&rft.date=2018-01-01&rft.eissn=1873-4316&rft.volume=19&rft.issue=11&rft.spage=917&rft_id=info:doi/10.2174%2F1389201020666181126120401&rft_id=info%3Apmid%2F30474527&rft_id=info%3Apmid%2F30474527&rft.externalDocID=30474527 |