Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage

• Published in: Reproduction (Cambridge, England) Vol. 138; no. 5; pp. 783 - 791
• Main Authors: Toralová, Tereza, Šušor, Andrej, Němcová, Lucie, Kepková, Kateřina, Kaňka, Jiří
• Format: Journal Article
• Language: English
• Published: England BioScientifica 01.11.2009; BioScientifica Ltd; Society for Reproduction and Fertility
• Subjects: Animals; Blastocyst - cytology; Blastocyst - metabolism; Blastocyst - physiology; Cattle - embryology; Cattle - genetics; Cattle - physiology; Cell Cycle Checkpoints - genetics; Cell Cycle Checkpoints - physiology; Cell Division - genetics; Cell Division - physiology; Cells, Cultured; Chromosomal Proteins, Non-Histone - antagonists & inhibitors; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - metabolism; Cleavage Stage, Ovum - metabolism; Cleavage Stage, Ovum - physiology; Embryonic Development - genetics; Embryonic Development - physiology; Female; Fertilization in Vitro - veterinary; Gene Expression Regulation, Developmental; Gene Silencing - physiology; Microfilament Proteins - antagonists & inhibitors; Microfilament Proteins - genetics; Microfilament Proteins - metabolism

Identification of genes that are important for normal preimplantation development is essential for understanding the basics of early mammalian embryogenesis. In our previous study, we have shown that CENPF (mitosin) is differentially expressed during preimplantation development of bovine embryos. CE...