Dehydroepiandrosterone administration prevents the oxidative damage induced by acute hyperglycemia in rats

• Published in: Journal of endocrinology Vol. 155; no. 2; pp. 233 - 240
• Main Authors: Aragno, M, Brignardello, E, Tamagno, E, Gatto, V, Danni, O, Boccuzzi, G
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Colchester BioScientifica 01.11.1997; Portland Press
• Subjects: Analysis of Variance; Animals; Antioxidants - pharmacology; Biological and medical sciences; Brain - drug effects; Brain - metabolism; Dehydroepiandrosterone - pharmacology; Glucose; Hormones. Endocrine system; Hyperglycemia - metabolism; Kidney - drug effects; Kidney - metabolism; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Male; Medical sciences; Models, Biological; Pharmacology. Drug treatments; Rats; Rats, Wistar; Vitamin E - pharmacology; Oxidative stress; Oxygen; Intraperitoneal administration; Rat; Rodentia; Lipids; Free radical; Prevention; Vertebrata; Dehydroepiandrosterone; Hyperglycemia; Experimental disease; Mammalia; Animal; Peroxidation
• ISSN: 0022-0795; 1479-6805
• DOI: 10.1677/joe.0.1550233

Free radical overproduction contributes to tissue damage induced by acute hyperglycemia. Dehydroepiandrosterone, which has recently been found to have antioxidant properties, was administered i.p. to rats at different doses (10, 50 or 100 mg/kg body weight) 3 h before treatment with dextrose (5 g/kg). Lipid peroxidation was evaluated on liver, brain and kidney homogenates, measuring both steady-state concentrations of thiobarbituric acid reactive substances, and fluorescent chromolipids, evaluated as hydroxynonenal adducts. Formation of thiobarbituric acid reactive substances was significantly higher in hyperglycemic than in normoglycemic animals. Three hours (but not 1 h) dehydroepiandrosterone-pretreatment protected tissues against lipid peroxidation induced by dextrose; both thiobarbituric acid reactive substances and hydroxynonenal adducts in liver, kidney and brain homogenates were significantly lower in dehydroepiandrosterone-pretreated animals. Dehydroepiandrosterone did not modify the cytosolic level of antioxidants, such as alpha-tocopherol or glutathione, nor the activities of glutathione peroxidase, reductase or transferase. The results of this study indicate that the 'in vivo' administration of dehydroepiandrosterone increases tissue resistance to lipid peroxidation triggered by acute hyperglycemia.