Development and Characterization of 11 Polymorphic Microsatellite Markers in Tapiscia sinensis (Staphyleaceae)

• Published in: Applications in plant sciences Vol. 1; no. 12; pp. 1300051 - n/a
• Main Authors: Zhang, Pengfei, Wang, Zhiguang, Liu, Yaling, Tian, Hua, Yao, Xiaohong, Zhang, Jinju
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01.12.2013; John Wiley & Sons, Inc; Wiley
• Subjects: alleles; Biotechnology; Cloning; conservation genetics; Deoxyribonucleic acid; DNA; Endangered & extinct species; Endangered populations; Endangered species; Flowers & plants; Gene polymorphism; Genetic diversity; Genetic markers; Genetic structure; genetic variation; genomic libraries; Heterozygosity; loci; microsatellite; microsatellite repeats; Microsatellites; Plant sciences; Population; Population genetics; PRIMER NOTES; Primers; Staphyleaceae; Tapiscia; Tapiscia sinensis; trees; United States > US; China; microsatellite; Staphyleaceae; Tapiscia sinensis; conservation genetics

Premise of the study: We developed and characterized five polymorphic microsatellite loci from an AC-enriched genomic library of a rare tree, Tapiscia sinensis, and six compound microsatellite primers using a dual-suppression-PCR–based approach, to investigate geographical genetic structure. Methods...