Analysis of the interaction between human steroid 21-hydroxylase and various monoclonal antibodies using comparative structural modelling

• Published in: European journal of endocrinology Vol. 153; no. 6; pp. 949 - 961
• Main Authors: Miguel, Ricardo Núñez, Chen, Shu, Nikfarjam, Laleh, Kominami, Shiro, Carpenter, Byron, Dal Pra, Chiara, Betterle, Corrado, Zanchetta, Renato, Nakamatsu, Takashi, Powell, Michael, Hewer, Rachel, Blundell, Tom L, Smith, Bernard Rees, Furmaniak, Jadwiga
• Format: Journal Article
• Language: English
• Published: Colchester European Society of Endocrinology 01.12.2005; Portland Press
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antigen-Antibody Reactions; Autoantibodies - immunology; Binding Sites; Binding Sites, Antibody; Biological and medical sciences; Endocrinopathies; Epitopes; Experimental Studies; Fundamental and applied biological sciences. Psychology; Humans; Medical sciences; Mice; Models, Immunological; Models, Molecular; Sequence Homology, Nucleic Acid; Steroid 21-Hydroxylase - genetics; Steroid 21-Hydroxylase - immunology; Steroid 21-Hydroxylase - metabolism; Vertebrates: endocrinology; Human; Enzyme; Oxidoreductases; Monoclonal antibody; Endocrinology; Comparative study; Steroid 21-monooxygenase

Objective: To study the interaction between human steroid 21-hydroxylase (21-OH) and monoclonal antibodies (MAbs) to 21-OH directed to 3 different epitopes recognised by 21-OH autoantibodies characteristic of autoimmune Addison’s disease. Design: Build comparative structural models of 21-OH, 21-OH M...