Diversity of Rickettsiae in Feeding and Questing Ticks Collected From a Malaysian Forest Reserve Area

• Published in: Journal of medical entomology Vol. 56; no. 2; pp. 547 - 552
• Main Authors: Kho, Kai Ling, Tan, Poai Ean, Tay, Sun Tee
• Format: Journal Article
• Language: English
• Published: US Entomological Society of America 25.02.2019; Oxford University Press
• Subjects: Animals; Biodiversity; Explosions; Feeding; Female; forest reserve; Forest reserves; Forests; Gene sequencing; Genes; Genetic relationship; Health aspects; Identification and classification; Indigenous peoples; Local communities; Malaysia; Male; Membrane proteins; Protein A; Questing; Rats; Rickettsia; Rickettsiae; Rickettsieae - genetics; Rickettsieae - isolation & purification; Species diversity; Ticks; Ticks - microbiology; VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION; Vectors; Virus-vector relationships; Malaysia; rickettsia; Malaysia; ticks; forest reserve
• DOI: 10.1093/jme/tjy168
• ISSN: 0022-2585

High seropositivity to Rickettsia conorii and Rickettsia felis has been reported in Malaysian indigenous community living in settlements adjacent to forest areas. The current study was conducted to determine the type and distribution of rickettsiae in feeding and questing ticks that were collected from a forest reserve area at Kuala Lompat in Pahang, Malaysia. Using PCR assays targeting citrate synthase (gltA), outer membrane protein A (ompA) and B (ompB) genes, rickettsiae were detected from approximately one-third of 98 ticks (mainly Dermacentor and Haemaphysalis spp.) collected from the forest reserve. BLAST analysis reveals the predominance of Rickettsia sp. RF2125 in both feeding and questing ticks and Rickettsia sp. TCM1 in the questing ticks. Sequences exhibiting close genetic relationship with Rickettsia raoultii, Rickettsia tamurae, Rickettsia heilongjiangensis, and Rickettsia asiatica were also detected from the ticks. This study highlights the diversity of rickettsial species and potential tick vectors which may contribute to the high seropositivity observed among the local communities.