in vitro larval immersion microassay for identifying and characterizing candidate acaricides

• Published in: Journal of medical entomology Vol. 41; no. 6; pp. 1034 - 1042
• Main Authors: White, W.H, Plummer, P.R, Kemper, C.J, Miller, R.J, Davey, R.B, Kemp, D.H, Hughes, S, Smith, C.K. II, Gutierrez, J.A
• Format: Journal Article
• Language: English
• Published: England 01.11.2004
• Subjects: acaricide resistance; acaricides; Amblyomma americanum; animal models; Animals; bioassays; Biological Assay - methods; Chlorpyrifos; dose response; Insect Control - methods; Insecticides - classification; Insecticides - toxicity; Larva - drug effects; larvae; mortality; Permethrin; potency; Pyrazoles; screening; speed of kill; tick control; tick models; ticks; Ticks - growth & development
• DOI: 10.1603/0022-2585-41.6.1034
• ISSN: 0022-2585

We have optimized a larval immersion microassay (LIM) that offers superior sensitivity, flexibility to accommodate multiple formulations, and a robust capability for rapidly screening many compounds with a minimal requirement of test article for evaluation. Dose-response studies were conducted for representative members from the organophosphate, pyrethroid, pyrazole, carbamate, macrocyclic lactone, and formamidine chemistries against Amblyomma americanum (L.). Time-response experiments revealed that permethrin was the most rapid acting, whereas fipronil had the slowest speed-of-kill against A. americanum. Comparison of drug susceptibility profiles between multiple ixodid ticks suggests that A. americanum is an effective model for predicting compound potency against Boophilus spp. in this bioassay. The LIM is suitable for the identification and characterization of active molecules from small- and medium-sized compound or natural product libraries, and it can be a useful tool to prioritize molecules for further in vivo testing in animal models.