Effect of PUFAs Oral Administration on the Amount of Apoptotic Caspases Enzymes in Gastric Cancer Patients Undergoing Chemotherapy
Gastric cancer is the fourth most common cancer and the second cause of death in the world. According to the studies, the gastric cancer is relatively sensitive to chemotherapy. The aim of this study was to investigate the association of oral administer PUFAs with Caspase enzymes in patients with ga...
Saved in:
Published in | Anti-cancer agents in medicinal chemistry Vol. 17; no. 1; p. 93 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
2017
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Gastric cancer is the fourth most common cancer and the second cause of death in the world. According to the studies, the gastric cancer is relatively sensitive to chemotherapy. The aim of this study was to investigate the association of oral administer PUFAs with Caspase enzymes in patients with gastric cancer under chemotherapy.
This study was a Clinical Trial in which the target group consisted of the patients recognized with gastric cancer for the first time and cured under chemotherapy. Thirty-four patients were selected and categorized randomly into two groups. The case group included the patients taking PUFAs along with chemotherapeutic agent. In these patients, chemotherapy started with Cis-Platin plus PUFAs supplement in the scale of 3600 mg daily and in three courses. In control group, the individuals were under the same chemotherapy protocol without PUFAs. Biopsy samples from tumor were taken from the patients before and after chemotherapy. Levels of mRNA and protein expression of caspase 3, 8, 9 were measured in biopsy samples by Real-Time PCR and Frozen Section methods. The levels of apoptosis were determined using DNA-damage colorimetric assay.
In the case group, caspase 3 showed a significant increase in both gene and protein expression levels after administration of PUFAs supplement in comparison with those of the control group (p=0.006 for gene, p=0.001 for protein). PUFAs induced caspase-9 gene expression level in these patients (p<0.0001). Caspase-9 protein level also revealed a marked elevation when PUFAs were administered along with chemotherapeutic agent (p<0.0001). DNA damage in gastric tissue from the patients under PUFAs treatment plus Cis-Platin was significantly higher than that of control group (p=0.003). PUFAs showed no significant changes in caspase-8 both at the gene and protein levels in the patients.
According to the results of present study, it appears that oral administration of PUFAs can elevate the efficacy of chemotherapy agent in individuals' mitochondria-dependent apoptosis. As PUFAs enhances caspase-3 and 9 genes expression levels, which is an important induce the mitochondrial dependent apoptosis process. The study was registered in Iran clinical trials registry center under No. IRCT2014031016922N1. |
---|---|
AbstractList | Gastric cancer is the fourth most common cancer and the second cause of death in the world. According to the studies, the gastric cancer is relatively sensitive to chemotherapy. The aim of this study was to investigate the association of oral administer PUFAs with Caspase enzymes in patients with gastric cancer under chemotherapy.
This study was a Clinical Trial in which the target group consisted of the patients recognized with gastric cancer for the first time and cured under chemotherapy. Thirty-four patients were selected and categorized randomly into two groups. The case group included the patients taking PUFAs along with chemotherapeutic agent. In these patients, chemotherapy started with Cis-Platin plus PUFAs supplement in the scale of 3600 mg daily and in three courses. In control group, the individuals were under the same chemotherapy protocol without PUFAs. Biopsy samples from tumor were taken from the patients before and after chemotherapy. Levels of mRNA and protein expression of caspase 3, 8, 9 were measured in biopsy samples by Real-Time PCR and Frozen Section methods. The levels of apoptosis were determined using DNA-damage colorimetric assay.
In the case group, caspase 3 showed a significant increase in both gene and protein expression levels after administration of PUFAs supplement in comparison with those of the control group (p=0.006 for gene, p=0.001 for protein). PUFAs induced caspase-9 gene expression level in these patients (p<0.0001). Caspase-9 protein level also revealed a marked elevation when PUFAs were administered along with chemotherapeutic agent (p<0.0001). DNA damage in gastric tissue from the patients under PUFAs treatment plus Cis-Platin was significantly higher than that of control group (p=0.003). PUFAs showed no significant changes in caspase-8 both at the gene and protein levels in the patients.
According to the results of present study, it appears that oral administration of PUFAs can elevate the efficacy of chemotherapy agent in individuals' mitochondria-dependent apoptosis. As PUFAs enhances caspase-3 and 9 genes expression levels, which is an important induce the mitochondrial dependent apoptosis process. The study was registered in Iran clinical trials registry center under No. IRCT2014031016922N1. |
Author | Aghaei, Mahmud Movahedian, Ahmad Mirza-Aghazade, Ahmad Somi, Mohammad-Hossein Samadi, Naser Dolatkhah, Homayun Esfahani, Ali |
Author_xml | – sequence: 1 givenname: Homayun surname: Dolatkhah fullname: Dolatkhah, Homayun – sequence: 2 givenname: Ahmad surname: Movahedian fullname: Movahedian, Ahmad organization: Clinical Biochemistry, PhD, Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutics Sciences, Isfahan University of Medical Sciences, Isfahan, I. R. Iran – sequence: 3 givenname: Mohammad-Hossein surname: Somi fullname: Somi, Mohammad-Hossein – sequence: 4 givenname: Mahmud surname: Aghaei fullname: Aghaei, Mahmud – sequence: 5 givenname: Naser surname: Samadi fullname: Samadi, Naser – sequence: 6 givenname: Ahmad surname: Mirza-Aghazade fullname: Mirza-Aghazade, Ahmad – sequence: 7 givenname: Ali surname: Esfahani fullname: Esfahani, Ali |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27198984$$D View this record in MEDLINE/PubMed |
BookMark | eNo1kM1OwzAQhC0Eoj_wCsg8QMDrn8Q-RlFbkCq1B3qunMRugxo7it1DOfLkWAVWK81IM_sddoZunXcGoWcgLxQK_gqyAEFJDnkayEnyAEwQdoOmKROZUIpO0CyET0K4SMV7NKEFKKkkn6LvhbWmidhbvN0ty4A3oz7hsu0714U46th5h9PGo8Fl78_uWi0HP0QfuwZXOgw6mIAX7uvSJ-0cXul0ec1cY0a8TRDjYsA715rx4Dt3wNXR9D4xRz1cHtCd1adgHv90jnbLxUf1lq03q_eqXGc15xCzVhrCCwZgJZhWsUZJ0yhtBEiraiZokTPSikZSnhqssVzVWqnaFLllwIDO0dMvdzjXvWn3w9j1erzs_59BfwCxnWRJ |
CitedBy_id | crossref_primary_10_3892_ol_2018_8979 crossref_primary_10_34172_mj_2020_035 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.2174/1871520616666160520113503 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Pharmacy, Therapeutics, & Pharmacology |
EISSN | 1875-5992 |
ExternalDocumentID | 27198984 |
Genre | Clinical Trial Journal Article |
GroupedDBID | --- .5. 0R~ 23M 36B 4.4 53G 5GY AAEGP AAVXF ABEEF ABJNI ABVDF ACGFS ACITR AENEX AFUQM AGJNZ ALMA_UNASSIGNED_HOLDINGS ANTIV C1A CGR CS3 CUY CVF EBS ECM EIF EJD F5P GH2 HZ~ IPNFZ KCGFV NPM O9- P2P RIG |
ID | FETCH-LOGICAL-b441t-d8e047311f81ed93c98ec9ae518f9b3527630d5c824f813cf49ba99be76f31312 |
IngestDate | Thu May 23 23:22:25 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-b441t-d8e047311f81ed93c98ec9ae518f9b3527630d5c824f813cf49ba99be76f31312 |
PMID | 27198984 |
ParticipantIDs | pubmed_primary_27198984 |
PublicationCentury | 2000 |
PublicationDate | 2017-00-00 |
PublicationDateYYYYMMDD | 2017-01-01 |
PublicationDate_xml | – year: 2017 text: 2017-00-00 |
PublicationDecade | 2010 |
PublicationPlace | Netherlands |
PublicationPlace_xml | – name: Netherlands |
PublicationTitle | Anti-cancer agents in medicinal chemistry |
PublicationTitleAlternate | Anticancer Agents Med Chem |
PublicationYear | 2017 |
SSID | ssj0045061 |
Score | 2.214954 |
Snippet | Gastric cancer is the fourth most common cancer and the second cause of death in the world. According to the studies, the gastric cancer is relatively... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 93 |
SubjectTerms | Adenocarcinoma - drug therapy Adenocarcinoma - genetics Adenocarcinoma - pathology Administration, Oral Aged Aged, 80 and over Antineoplastic Agents - administration & dosage Antineoplastic Agents - therapeutic use Apoptosis - drug effects Caspase 3 - genetics Caspase 8 - genetics Caspase 9 - genetics Caspases - analysis Caspases - genetics DNA Damage - drug effects Fatty Acids, Omega-3 - administration & dosage Fatty Acids, Omega-3 - therapeutic use Fatty Acids, Omega-6 - administration & dosage Fatty Acids, Omega-6 - therapeutic use Fatty Acids, Unsaturated - administration & dosage Fatty Acids, Unsaturated - therapeutic use Female Humans Male Middle Aged Stomach - drug effects Stomach - metabolism Stomach - pathology Stomach Neoplasms - drug therapy Stomach Neoplasms - genetics Stomach Neoplasms - pathology Up-Regulation - drug effects |
Title | Effect of PUFAs Oral Administration on the Amount of Apoptotic Caspases Enzymes in Gastric Cancer Patients Undergoing Chemotherapy |
URI | https://www.ncbi.nlm.nih.gov/pubmed/27198984 |
Volume | 17 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Li9swEBbZFkovS9_vokLZS9ZtZFmxdTTLbkMh2z0ksLdFluU4FNuhcRayx_7B_qXOSLbzaLe0hSCCXjGeL6OZ0TwIec8jLZSWqScTPvRQZPAiA393I7KAm2TImUE75Ph8OJoGny_FZa_3Y8traVUnH_TNb-NK_oeq0Ad0xSjZf6Bstyl0wHegL7RAYWj_isZN6mF0Y5uexcv-F4y2302H21wG9OMCi0I4ubNa1BXmaT1RwE6WZtk_LW_WhfXM6n9SWMgDxwAN3zCD_9zGwNn6SLPKegnkpmjitnbuhOOynnvarVMzuwo2bC7vMQ1JW1uuE55Bra6_5spadkZVodarDqrj6lrlGNXiUhzkhUo7Y1BVWBeEcZWrAvq9ERz0Zt4tjWe5Mm6GyotVum3YYOGWYcM4Zgy6lCek3OXW4S-odKzXFVrcPxFQ40LjBOiFwgfZBbQ1aND5hzEuBjtrgLiLwkLFD9GVzNWu-_PoXrLuduiAHIQRMtxzNB45wSAQ8Pv3yLvmqT7e-kyYoLrZZ0_ZsULP5AE5bLQVGjvoPSQ9Uz4iRxcu3fn6mE420XvLY3pELzaJ0NePyXeHT1pl1OKTIj7pLj4pfABL1OETp3b4pC0-aYNPOi9pg0_q8ElbfNINPuk2Pp-Q6dnp5GTkNTU_vAQE89pLIzMIQs5YFjGTSq5lZLRURrAoA3YifDgPB6nQkR_ADK6zQCZKysSEw4wzzvyn5E5ZleY5oUykLBgoE-g0DXQoFId9pZFw5mjJVPiCPHPv9mrhErtctW_95a0jr8j9DVRfk7sZcBLzBsTSOnlrif0Twc2PUQ |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Effect+of+PUFAs+Oral+Administration+on+the+Amount+of+Apoptotic+Caspases+Enzymes+in+Gastric+Cancer+Patients+Undergoing+Chemotherapy&rft.jtitle=Anti-cancer+agents+in+medicinal+chemistry&rft.au=Dolatkhah%2C+Homayun&rft.au=Movahedian%2C+Ahmad&rft.au=Somi%2C+Mohammad-Hossein&rft.au=Aghaei%2C+Mahmud&rft.date=2017-01-01&rft.eissn=1875-5992&rft.volume=17&rft.issue=1&rft.spage=93&rft_id=info:doi/10.2174%2F1871520616666160520113503&rft_id=info%3Apmid%2F27198984&rft_id=info%3Apmid%2F27198984&rft.externalDocID=27198984 |