Discovery of a possible role of asprosin in ovarian follicular function
Asprosin is a novel fasting-induced protein encoded by fibrillin-1 (FBN1) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with insulin resistance and polycystic ovarian syndrome in humans. To characterize mRNA abundance of FBN1, FURIN, and the presumed asprosin receptor, o...
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Published in | Journal of molecular endocrinology Vol. 66; no. 1; pp. 35 - 44 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Bioscientifica Ltd
01.01.2021
Society for Endocrinology & BioScientifica Ltd |
Subjects | |
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Abstract | Asprosin is a novel fasting-induced protein encoded by fibrillin-1 (FBN1) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with insulin resistance and polycystic ovarian syndrome in humans. To characterize mRNA abundance of FBN1, FURIN, and the presumed asprosin receptor, olfactory receptor family 4 subfamily M member 1 (OR4M1) in granulosa (GC) and theca cells (TC), and identify hormones regulating FBN1 mRNA expression, GC and TC from small (1–5 mm; SM) and large (>8 mm; LG) follicles were collected from ovaries of heifers obtained at an abattoir and used for real-time PCR gene expression analysis or in vitro evaluation of hormone regulation and asprosin effects. SMTC had 151-fold greater (P < 0.05) FBN1 mRNA abundance than SMGC, and LGTC had 50-fold greater FBN1 mRNA than LGGC. In contrast, OR4M1 mRNA was 81-fold greater in SMGC than LGGC and did not differ from SMTC, but LGTC had 9-fold greater OR4M1 mRNA than LGGC. FURIN mRNA was 2.6-fold greater in SMTC than SMGC, but did not differ among follicular sizes. In cultured TC, leptin, insulin, LH, IGF1 and steroids did not affect FBN1 mRNA, but TGFB1 increased (P < 0.05) FBN1 mRNA by 2.2-fold; EGF and FGFs increased FBN1 mRNA by 1.3- to 1.5-fold. Asprosin enhanced LH-induced TC androstenedione production, reduced IGF1-induced TC proliferation, and had no effect on progesterone production. Developmental regulation of FBN1, FURIN and OR4M1 along with direct effects of asprosin on TC suggests that asprosin may be a novel regulator of ovarian follicular function. |
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AbstractList | Asprosin is a novel fasting-induced protein encoded by fibrillin-1 (
FBN1
) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with insulin resistance and polycystic ovarian syndrome in humans. To characterize mRNA abundance of
FBN1
, FURIN, and the presumed asprosin receptor, olfactory receptor family 4 subfamily M member 1 (
OR4M1
) in granulosa (GC) and theca cells (TC), and identify hormones regulating
FBN1
mRNA expression, GC and TC from small (1–5 mm; SM) and large (>8 mm; LG) follicles were collected from ovaries of heifers obtained at an abattoir and used for real-time PCR gene expression analysis or
in vitro
evaluation of hormone regulation and asprosin effects. SMTC had 151-fold greater (
P <
0.05)
FBN1
mRNA abundance than SMGC, and LGTC had 50-fold greater
FBN1
mRNA than LGGC. In contrast,
OR4M1
mRNA was 81-fold greater in SMGC than LGGC and did not differ from SMTC, but LGTC had 9-fold greater
OR4M1
mRNA than LGGC. FURIN mRNA was 2.6-fold greater in SMTC than SMGC, but did not differ among follicular sizes. In cultured TC, leptin, insulin, LH, IGF1 and steroids did not affect
FBN1
mRNA, but TGFB1 increased (
P
< 0.05)
FBN1
mRNA by 2.2-fold; EGF and FGFs increased
FBN1
mRNA by 1.3- to 1.5-fold. Asprosin enhanced LH-induced TC androstenedione production, reduced IGF1-induced TC proliferation, and had no effect on progesterone production. Developmental regulation of
FBN1
, FURIN and
OR4M1
along with direct effects of asprosin on TC suggests that asprosin may be a novel regulator of ovarian follicular function. Asprosin is a novel fasting-induced protein encoded by fibrillin-1 (FBN1) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with insulin resistance and polycystic ovarian syndrome in humans. To characterize mRNA abundance of FBN1, FURIN, and the presumed asprosin receptor, olfactory receptor family 4 subfamily M member 1 (OR4M1) in granulosa (GC) and theca cells (TC), and identify hormones regulating FBN1 mRNA expression, GC and TC from small (1–5 mm; SM) and large (>8 mm; LG) follicles were collected from ovaries of heifers obtained at an abattoir and used for real-time PCR gene expression analysis or in vitro evaluation of hormone regulation and asprosin effects. SMTC had 151-fold greater (P < 0.05) FBN1 mRNA abundance than SMGC, and LGTC had 50-fold greater FBN1 mRNA than LGGC. In contrast, OR4M1 mRNA was 81-fold greater in SMGC than LGGC and did not differ from SMTC, but LGTC had 9-fold greater OR4M1 mRNA than LGGC. FURIN mRNA was 2.6-fold greater in SMTC than SMGC, but did not differ among follicular sizes. In cultured TC, leptin, insulin, LH, IGF1 and steroids did not affect FBN1 mRNA, but TGFB1 increased (P < 0.05) FBN1 mRNA by 2.2-fold; EGF and FGFs increased FBN1 mRNA by 1.3- to 1.5-fold. Asprosin enhanced LH-induced TC androstenedione production, reduced IGF1-induced TC proliferation, and had no effect on progesterone production. Developmental regulation of FBN1, FURIN and OR4M1 along with direct effects of asprosin on TC suggests that asprosin may be a novel regulator of ovarian follicular function. |
Author | Batalha, Isadora Spicer, Leon J Maylem, Excel Rio S Schutz, Luis F |
AuthorAffiliation | Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada, USA Department of Animal and Food Sciences, Oklahoma State University, Stillwater, Oklahoma, USA |
AuthorAffiliation_xml | – name: Department of Animal and Food Sciences, Oklahoma State University, Stillwater, Oklahoma, USA – name: Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada, USA |
Author_xml | – sequence: 1 givenname: Excel Rio S surname: Maylem fullname: Maylem, Excel Rio S organization: Department of Animal and Food Sciences, Oklahoma State University, Stillwater, Oklahoma, USA – sequence: 2 givenname: Leon J surname: Spicer fullname: Spicer, Leon J email: Leon.spicer@okstate.edu organization: Department of Animal and Food Sciences, Oklahoma State University, Stillwater, Oklahoma, USA – sequence: 3 givenname: Isadora surname: Batalha fullname: Batalha, Isadora organization: Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada, USA – sequence: 4 givenname: Luis F surname: Schutz fullname: Schutz, Luis F organization: Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada, USA |
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Snippet | Asprosin is a novel fasting-induced protein encoded by fibrillin-1 (FBN1) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with... Asprosin is a novel fasting-induced protein encoded by fibrillin-1 ( FBN1 ) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with... |
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SubjectTerms | Abattoirs Androstenedione Fibrillin Follicles Furin Gene expression Guanylate cyclase Insulin Insulin resistance Insulin-like growth factor I Leptin Luteinizing hormone Ovaries Polycystic ovary syndrome Progesterone Steroid hormones Theca Transforming growth factor-b1 |
Title | Discovery of a possible role of asprosin in ovarian follicular function |
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