Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

• Published in: Microbial cell factories Vol. 14; no. 1; p. 1
• Main Authors: Gmeiner, Christoph, Saadati, Amirhossein, Maresch, Daniel, Krasteva, Stanimira, Frank, Manuela, Altmann, Friedrich, Herwig, Christoph, Spadiut, Oliver
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.01.2015; BioMed Central
• Subjects: Batch Cell Culture Techniques; Chromatography, High Pressure Liquid; Electrophoresis; Glycopeptides - analysis; Glycosylation; Horseradish Peroxidase - chemistry; Horseradish Peroxidase - genetics; Horseradish Peroxidase - metabolism; Hydrogen-Ion Concentration; Methanol - metabolism; Oxygen Consumption; Pichia - genetics; Pichia - metabolism; Plant Proteins - chemistry; Plant Proteins - genetics; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Spectrometry, Mass, Electrospray Ionization; Temperature

Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to its capability of glycosylation. However, N-linked glycans on recombinant proteins get hypermannosylated, causing problems in subsequent unit operations and medical applications. Hypermannosylation is...