Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species
Wild populations of Caiman latirostris are subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management,...
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| Published in | Amphibia-reptilia Vol. 36; no. 2; pp. 175 - 180 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Brill
01.01.2015
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| Abstract | Wild populations of Caiman latirostris are subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management, which may be obtained from analysis of molecular markers. Some microsatellites have been previously isolated in this species, but due to some difficulties in using them, we obtained new ones using Next Generation Sequencing approach. This study reports eight new microsatellites for C. latirostris and tests their utility in a related species, Caiman yacare, with successful application in population genetics and mating systems studies. In addition, we shared data about a novel and fast bioinformatics tool to find microsatellites and to design their corresponding primers. |
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| AbstractList | Wild populations of Caiman latirostris are subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management, which may be obtained from analysis of molecular markers. Some microsatellites have been previously isolated in this species, but due to some difficulties in using them, we obtained new ones using Next Generation Sequencing approach. This study reports eight new microsatellites for C. latirostris and tests their utility in a related species, Caiman yacare, with successful application in population genetics and mating systems studies. In addition, we shared data about a novel and fast bioinformatics tool to find microsatellites and to design their corresponding primers. Wild populations of Caiman latirostris are subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management, which may be obtained from analysis of molecular markers. Some microsatellites have been previously isolated in this species, but due to some difficulties in using them, we obtained new ones using Next Generation Sequencing approach. This study reports eight new microsatellites for C. latirostris and tests their utility in a related species, Caiman yacare , with successful application in population genetics and mating systems studies. In addition, we shared data about a novel and fast bioinformatics tool to find microsatellites and to design their corresponding primers. |
| Author | Saidman, Beatriz Ofelia Amavet, Patricia Susana Siroski, Pablo Ariel Larriera, Alejandro Rueda, Eva Carolina |
| Author_xml | – sequence: 1 givenname: Patricia Susana surname: Amavet fullname: Amavet, Patricia Susana – sequence: 2 givenname: Eva Carolina surname: Rueda fullname: Rueda, Eva Carolina – sequence: 3 givenname: Pablo Ariel surname: Siroski fullname: Siroski, Pablo Ariel – sequence: 4 givenname: Alejandro surname: Larriera fullname: Larriera, Alejandro – sequence: 5 givenname: Beatriz Ofelia surname: Saidman fullname: Saidman, Beatriz Ofelia |
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| Cites_doi | 10.1007/s10592-008-9600-7 10.1007/s12686-010-9221-6 10.1177/117693430500100003 10.1111/j.1471-8286.2004.00684.x 10.1163/156853812X624423 10.1007/s12686-013-9932-6 10.1002/jez.10190 10.1670/0022-1511(2007)41[294:AOTPSO]2.0.CO;2 10.1002/jez.430 10.1186/1471-2164-14-176 10.1111/j.1755-0998.2009.02750.x 10.1016/j.biocon.2010.05.011 10.4238/2014.July.25.2 10.1002/ece3.764 10.1163/15685381-00002861 10.1007/s10592-008-9601-6 10.1002/jez.496 10.1002/jez.523 10.1590/S1415-47572006000100015 |
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| SubjectTerms | Argentina bioinformatics Caiman latirostris Caiman yacare genetic markers high-throughput nucleotide sequencing mating systems meat microsatellite repeats |
| Title | Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species |
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