Probing the Spatial Dependence of the Emission Spectrum of Single Human Retinal Lipofuscin Granules Using Near-field Scanning Optical Microscopy

• Published in: Photochemistry and photobiology Vol. 74; no. 2; pp. 364 - 368
• Main Authors: Haralampus-Grynaviski, Nicole M., Lamb, Laura E., Simon, John D., Krogmeier, Jeffrey R., Dunn, Robert C., Pawlak, Anna, Rózanowska, Malgorzata, Sarna, Tadeusz, Burke, Janice M.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.08.2001
• Subjects: Humans; Lipofuscin - chemistry; Microscopy, Fluorescence - methods; Photochemistry; PHOTOSENSORY AND CIRCADIAN BIOLOGY; Pigment Epithelium of Eye - chemistry; Spectrophotometry

The emission spectra of single lipofuscin granules are examined using spectrally resolved confocal microscopy and near-field scanning optical microscopy (NSOM). The emission spectrum varies among the granules examined revealing that individual granules are characterized by different distributions of...