Probing the Spatial Dependence of the Emission Spectrum of Single Human Retinal Lipofuscin Granules Using Near-field Scanning Optical Microscopy

The emission spectra of single lipofuscin granules are examined using spectrally resolved confocal microscopy and near-field scanning optical microscopy (NSOM). The emission spectrum varies among the granules examined revealing that individual granules are characterized by different distributions of...

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Published inPhotochemistry and photobiology Vol. 74; no. 2; pp. 364 - 368
Main Authors Haralampus-Grynaviski, Nicole M., Lamb, Laura E., Simon, John D., Krogmeier, Jeffrey R., Dunn, Robert C., Pawlak, Anna, Rózanowska, Malgorzata, Sarna, Tadeusz, Burke, Janice M.
Format Journal Article
LanguageEnglish
Published United States Blackwell Publishing Ltd 01.08.2001
Subjects
Humans
Lipofuscin - chemistry
Microscopy, Fluorescence - methods
Photochemistry
PHOTOSENSORY AND CIRCADIAN BIOLOGY
Pigment Epithelium of Eye - chemistry
Spectrophotometry
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Summary:The emission spectra of single lipofuscin granules are examined using spectrally resolved confocal microscopy and near-field scanning optical microscopy (NSOM). The emission spectrum varies among the granules examined revealing that individual granules are characterized by different distributions of fluorophores. The range of spectra observed is consistent with in vivo spectra of human retinal pigment epithelium cells. NSOM measurements reveal that the shape of the spectrum does not vary with position within the emissive regions of single lipofuscin granules. These results suggest that the relative distribution of fluorophores within the emissive regions of an individual granule is homogeneous on the spatial scale ∼150 nm.
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ISSN:0031-8655
1751-1097
DOI:10.1562/0031-8655(2001)074<0364:PTSDOT>2.0.CO;2