Molecular characterization of pear 14-3-3b gene regulated during fruit development

• Published in: Canadian journal of plant science Vol. 96; no. 3; pp. 433 - 438
• Main Authors: Shi, Haiyan, Yujing Zhao, Xuemin An, Yuxing Zhang
• Format: Journal Article
• Language: English
• Published: Canadian Science Publishing 01.06.2016
• Subjects: 14-3-3; complementary DNA; fruit development; fruiting; gene expression; genes; introns; pear (Pyrus pyrifolia); pears; phylogeny; protein-protein interactions; proteins; Pyrus pyrifolia; reverse transcriptase polymerase chain reaction; ripening; signal transduction
• ISSN: 1918-1833; 1918-1833
• DOI: 10.1139/CJPS-2015-0319

Plant 14-3-3 proteins (14-3-3s) are known to function in protein–protein interactions that mediate signal transduction pathways regulating many biological processes. The cDNA encoding putative 14-3-3 protein was isolated from pear (Pyrus pyrifolia) and designated Pp14-3-3b. Using the PCR amplification technique, the genomic clone corresponding to Pp14-3-3b was isolated and shown to contain six introns. Phylogenetic analysis clearly demonstrated that Pp14-3-3b was classified into the non-ɛ class of 14-3-3 superfamilies. Quantitative RT-PCR analysis indicated that the expression of the Pp14-3-3b gene was developmentally regulated in fruit. This study suggested that Pp14-3-3b might be involved in fruit ripening and the senescence of pear.