PURIFICATION, CHARACTERIZATION, AND IMMUNOLOCALIZATION OF A THIOREDOXIN REDUCTASE FROM ADULT FASCIOLA HEPATICA

• Published in: The Journal of parasitology Vol. 90; no. 2; pp. 205 - 211
• Main Authors: Maggioli, Gabriela, Piacenza, Lucía, Carambula, Beatriz, Carmona, Carlos
• Format: Journal Article
• Language: English
• Published: United States American Society of Parasitologists 01.04.2004
• Subjects: Animals; Antiserum; Aurothioglucose - pharmacology; Biochemistry; BIOCHEMISTRY-PHYSIOLOGY; Cattle; Chemical Fractionation; Chromatography, Affinity; Chromatography, Ion Exchange; Disulfides; Electrophoresis, Polyacrylamide Gel; enzyme activation; enzyme activity; enzyme inhibition; enzyme inhibitors; Enzyme Inhibitors - pharmacology; Enzymes; Fasciola hepatica; Fasciola hepatica - enzymology; Fluorescent Antibody Technique; Immunoblotting; immunochemistry; Immunoglobulin G - immunology; Insulin; liver flukes; oxidoreductases; Parasites; Parasitology; purification; Room temperature; Sulfates; Thioredoxin; Thioredoxin-Disulfide Reductase - antagonists & inhibitors; Thioredoxin-Disulfide Reductase - immunology; Thioredoxin-Disulfide Reductase - isolation & purification; Thioredoxin-Disulfide Reductase - metabolism; tissue distribution

Thioredoxin reductase (TrxR), an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases, was isolated from the deoxycholate-soluble extract of the common liver fluke, Fasciola hepatica. Purification to homogeneity of the 60-kDa enzyme from the adult worm was ach...