Characterization of LC-HCC fusion protein of botulinum neurotoxin type A

• Published in: Protein and peptide letters Vol. 18; no. 3; p. 295
• Main Authors: Singh, Manglesh Kumar, Dhaked, Ram Kumar, Singh, Padma, Gupta, Pallavi, Singh, Lokendra
• Format: Journal Article
• Language: English
• Published: Netherlands 01.03.2011
• Subjects: Amino Acid Sequence; Animals; Botulinum Toxins, Type A - chemistry; Botulinum Toxins, Type A - genetics; Botulinum Toxins, Type A - immunology; Botulinum Toxins, Type A - metabolism; Catalytic Domain; Cloning, Molecular; Clostridium botulinum - enzymology; Clostridium botulinum - genetics; Escherichia coli - genetics; Female; Gangliosides - metabolism; Genetic Vectors - genetics; Immunization; Mice; Molecular Sequence Data; Neutralization Tests; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - metabolism; Rats; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Recombinant Fusion Proteins - metabolism; Synaptic Vesicles - enzymology; Synaptic Vesicles - metabolism

Botulinum neurotoxins (BoNTs) are highly potent toxins that inhibit neurotransmitter release from peripheral cholinergic synapses. The gene for encoding the full length light chain with H(CC) (binding) domain of Clostridium botulinum neurotoxin A was synthesized and cloned into a bacterial expressio...