Deletion mutant comprising 198 residues of BoNT/A toxin receptor binding domain retained gt1b binding property but failed to induce protective antibody response in a mouse model

• Published in: Protein and peptide letters Vol. 19; no. 5; p. 530
• Main Authors: Singh, Manglesh Kumar, Gupta, Garima, Boopathi, Mannan, Gupta, Pallavi, Chauhan, Vinita, Tomar, Arvind, Singh, Lokendra, Dhaked, Ram Kumar
• Format: Journal Article
• Language: English
• Published: Netherlands 01.05.2012
• Subjects: Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Bacterial Vaccines - genetics; Bacterial Vaccines - immunology; Binding Sites; Botulinum Toxins, Type A - chemistry; Botulinum Toxins, Type A - genetics; Botulinum Toxins, Type A - immunology; Botulinum Toxins, Type A - metabolism; Cloning, Molecular; Clostridium botulinum - genetics; Clostridium botulinum - immunology; Enzyme-Linked Immunosorbent Assay; Escherichia coli - genetics; Escherichia coli - metabolism; Female; Gangliosides - metabolism; Histidine; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulin M - blood; Immunoglobulin M - immunology; Mice; Oligopeptides; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Sequence Deletion; Surface Plasmon Resonance

The most effective protection against toxin is inducing protective immune response through vaccination that will produce neutralizing antibodies. Antibodies will bind to and clear toxin from the circulation before it can enter nerve cells and block neurotransmission and can also be used for developm...