Regulation of the Dimerization and Activity of SARS-CoV-2 Main Protease through Reversible Glutathionylation of Cysteine 300

• Published in: bioRxiv
• Main Authors: Davis, David A, Bulut, Haydar, Shrestha, Prabha, Yaparla, Amulya, Jaeger, Hannah K, Hattori, Shin-Ichiro, Wingfield, Paul, Mitsuya, Hiroaki, Yarchoan, Robert
• Format: Journal Article Paper
• Language: English; Japanese
• Published: United States Cold Spring Harbor Laboratory 12.04.2021
• Edition: 1.2
• Subjects: Microbiology

SARS-CoV-2 encodes main protease (Mpro), an attractive target for therapeutic interventions. We show Mpro is susceptible to glutathionylation leading to inhibition of dimerization and activity. Activity of glutathionylated Mpro could be restored with reducing agents or glutaredoxin. Analytical studi...