IN VITRO STEROL AND STEROID SYNTHESIS BY OVARIES OF HYPOPHYSECTOMIZED IMMATURE RATS TREATED WITH HUMAN GONADOTROPHINS

• Published in: Acta endocrinologica (Copenhagen) Vol. 73; no. 2; pp. 321 - 334
• Main Authors: Bai, R U, Bischoff, K, Macome, J C, Diczfalusy, E
• Format: Journal Article
• Language: English
• Published: Denmark 01.06.1973
• Subjects: 17-Ketosteroids - biosynthesis; Acetates - metabolism; Androgens - biosynthesis; Androstenedione - biosynthesis; Animals; Carbon Isotopes; Cholesterol - biosynthesis; Chorionic Gonadotropin - pharmacology; Chromatography, Paper; Chromatography, Thin Layer; Estradiol - biosynthesis; Estrone - biosynthesis; Female; Follicle Stimulating Hormone - pharmacology; Gonadal Steroid Hormones - biosynthesis; Hypophysectomy; Luteinizing Hormone - pharmacology; Ovary - drug effects; Ovary - metabolism; Progesterone - biosynthesis; Rats; Sodium; Testosterone - biosynthesis; Tritium

ABSTRACT The in vitro conversion of sodium [ 1,2- 14C] acetate into sterols and steroids was studied following the incubation of groups of ovaries from hypophysectomized immature rats with human gonadotrophins. Tritium labelled internal standards were used to monitor procedural losses and sterols an...