Beta- and Gamma-Cytoplasmic Actins Are Required for Meiosis in Mouse Oocytes1
In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning of the cytoplasm results from migration of the meiotic spindle toward the cortex and requires actin filaments. However, the subcellular loca...
Saved in:
| Published in | Biology of reproduction Vol. 85; no. 5; pp. 1025 - 1039 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Society for the Study of Reproduction, Inc
01.11.2011
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning of the cytoplasm results from migration of the meiotic spindle toward the cortex and requires actin filaments. However, the subcellular localization and the role of the existing two cytoplasmic actin (CYA) isoforms, beta and gamma, have not been characterized. We show that beta- and gamma-CYA are differentially distributed in the maturing oocyte from late metaphase I as well as in preimplantation embryos. Gamma-CYA is preferentially enriched in oocyte cortices and is absent from all cell-cell contact areas from metaphase II until the blastocyst stage. Beta-CYA is enriched in contractile structures, at cytokinesis, at cell-cell contacts, and around the forming blastocoel. Alteration of beta- or gamma-CYA function by isoform-specific antibody microinjection suggests that gamma-CYA holds a major and specific role in the establishment and/or maintenance of asymmetry in meiosis I and in the maintenance of overall cortical integrity. In contrast, beta- and gamma-CYA, together, appear to participate in the formation and the cortical anchorage of the second meiotic spindle in waiting for fertilization. Finally, differences in gamma-CYA expression are amongst the earliest markers of cell fate determination in development. |
|---|---|
| AbstractList | In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning of the cytoplasm results from migration of the meiotic spindle toward the cortex and requires actin filaments. However, the subcellular localization and the role of the existing two cytoplasmic actin (CYA) isoforms, beta and gamma, have not been characterized. We show that beta- and gamma-CYA are differentially distributed in the maturing oocyte from late metaphase I as well as in preimplantation embryos. Gamma-CYA is preferentially enriched in oocyte cortices and is absent from all cell-cell contact areas from metaphase II until the blastocyst stage. Beta-CYA is enriched in contractile structures, at cytokinesis, at cell-cell contacts, and around the forming blastocoel. Alteration of beta- or gamma-CYA function by isoform-specific antibody microinjection suggests that gamma-CYA holds a major and specific role in the establishment and/or maintenance of asymmetry in meiosis I and in the maintenance of overall cortical integrity. In contrast, beta- and gamma-CYA, together, appear to participate in the formation and the cortical anchorage of the second meiotic spindle in waiting for fertilization. Finally, differences in gamma-CYA expression are amongst the earliest markers of cell fate determination in development. |
| Author | Huarte, Joachim Vassalli, Jean-Dominique Swetloff, Adam Conne, Béatrice Brockmann, Céline Dugina, Vera Challet, Ludivine Rey, Emmanuelle Chaponnier, Christine Nef, Serge |
| Author_xml | – sequence: 1 givenname: Céline surname: Brockmann fullname: Brockmann, Céline organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 2 givenname: Joachim surname: Huarte fullname: Huarte, Joachim organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 3 givenname: Vera surname: Dugina fullname: Dugina, Vera organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 4 givenname: Ludivine surname: Challet fullname: Challet, Ludivine organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 5 givenname: Emmanuelle surname: Rey fullname: Rey, Emmanuelle organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 6 givenname: Béatrice surname: Conne fullname: Conne, Béatrice organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 7 givenname: Adam surname: Swetloff fullname: Swetloff, Adam organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 8 givenname: Serge surname: Nef fullname: Nef, Serge organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 9 givenname: Christine surname: Chaponnier fullname: Chaponnier, Christine organization: University of Geneva Medical School, Geneva, Switzerland – sequence: 10 givenname: Jean-Dominique surname: Vassalli fullname: Vassalli, Jean-Dominique organization: University of Geneva Medical School, Geneva, Switzerland |
| BookMark | eNqN0MFLwzAUx_EgE9ymf4KQfyA1L2nS9jiHTmFjIHour-krRNZmJt1h_72VCV49vcPj-zt8Fmw2hIEYuweZgazMQ-PDIdIxhjYDgExWUGh7xeZgVCUKZcsZm0sprdDa6hu2SOlTSsi10nO2e6QRBceh5RvsexTr8xiOB0y9d3zlRj8kvorE3-jr5CO1vAuR78iH5BP3A9-FUyK-D-48UoJbdt3hIdHd712yj-en9_WL2O43r-vVVjTKGCu6wsgid84hoXFNgcZW1qkOG4VlS1BpLQFl5cpclaBzRzlpq3SLTeEkKL1k5rLrYkgpUlcfo-8xnmuQ9Y9J_WdSTyb1xWTq9KWb3pPhP6tv1-Vqkw |
| CitedBy_id | crossref_primary_10_1017_S1431927613001566 crossref_primary_10_1016_j_tox_2019_04_016 crossref_primary_10_1134_S0006297919060014 crossref_primary_10_17116_patol201779215_21 crossref_primary_10_1016_j_cellsig_2012_09_015 crossref_primary_10_1016_j_yexcr_2015_11_003 crossref_primary_10_1063_5_0096188 crossref_primary_10_1134_S0006297917060037 crossref_primary_10_1093_humrep_det261 crossref_primary_10_1134_S0006297912110053 crossref_primary_10_3390_ijms18091909 crossref_primary_10_1038_s41467_024_49427_2 crossref_primary_10_1002_cm_21647 crossref_primary_10_1017_S096719941500060X crossref_primary_10_1007_s00418_014_1199_9 crossref_primary_10_1080_09513590_2022_2147158 crossref_primary_10_1186_s12864_019_5687_0 crossref_primary_10_1038_s41467_017_01231_x crossref_primary_10_1371_journal_pone_0173709 crossref_primary_10_1038_s41467_019_12674_9 crossref_primary_10_1134_S0006297920090072 |
| Cites_doi | 10.1159/000086889 10.1016/S0960-9822(01)00639-X 10.1016/j.mce.2007.11.004 10.1016/0022-2836(78)90020-7 10.1002/mrd.20148 10.1016/0309-1651(81)90009-6 10.1016/S0925-4773(02)00350-7 10.1038/ng1382 10.1016/j.cub.2005.11.067 10.1016/j.bbrc.2004.05.040 10.1126/science.1073978 10.1042/BC20080003 10.1023/A:1008816308171 10.1038/ncb1788 10.1083/jcb.103.6.2787 10.1111/j.1432-0436.1987.tb01562.x 10.1159/000086896 10.1016/j.cub.2008.11.022 10.1073/pnas.94.17.9165 10.1523/JNEUROSCI.17-24-09565.1997 10.1091/mbc.9.9.2509 10.1016/j.cub.2008.08.044 10.1093/humupd/dmh036 10.1159/000132871 10.1016/j.devcel.2006.12.010 10.1016/S0378-5122(98)00070-X 10.1093/molehr/7.1.49 10.1002/cm.10043 10.1083/jcb.130.4.887 10.1177/003335490712200109 10.1242/jcs.041970 10.1006/dbio.1998.9117 10.1016/S0896-6273(00)80467-5 10.1002/mrd.20253 |
| ContentType | Journal Article |
| Copyright | 2011 by the Society for the Study of Reproduction, Inc. |
| Copyright_xml | – notice: 2011 by the Society for the Study of Reproduction, Inc. |
| DBID | AAYXX CITATION |
| DOI | 10.1095/biolreprod.111.091736 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Biology |
| DocumentTitleAlternate | ACTIN ISOFORMS IN OOCYTES AND EARLY EMBRYOS |
| EISSN | 1529-7268 |
| EndPage | 1039 |
| ExternalDocumentID | 10_1095_biolreprod_111_091736 |
| GroupedDBID | - 08R 0R 186 1TH 23N 2WC 3O- 3V. 48X 53G 5GY 5RE 5VS 5WD 7X7 88E 8FI 8FJ AABJS AABMN AACFU AAIMJ AAJQQ AAPBV AAPPN AAPQZ AAPSS AAUQX ABFLS ABPTD ABSAR ABSGY ABUWG ACFRR ACGFS ACNCT ACUFI ACUTJ ADBBV ADEIU ADGZP ADHKW ADIPN ADOYD ADRTK ADVEK AEDJY AELNO AELWJ AEMDU AENEX AENZO AETBJ AEWNT AFFNX AFFZL AFKRA AFULF AFXEN AGINJ AHMBA AIKOY AKPMI ALMA_UNASSIGNED_HOLDINGS APIBT ARIXL ASAOO ATDFG AYOIW AZQFJ BAWUL BAYMD BBAFP BBNVY BCRHZ BENPR BEYMZ BHONS BHPHI BPHCQ BSWAC BVXVI BYORX C1A C45 CAG CDBKE COF CS3 DAKXR DC7 DIK DPPUQ DU5 E3Z EBS EF EJD F5P FA8 FHSFR FYUFA GAUVT GJ GJXCC H13 HCIFZ H~9 IAO IHR INH INIJC JH KM KOP KQ8 KSN M1P M7P MBTAY MVM NLBLG O9- OBOKY ODMLO OHT OK1 OVD OWPYF P2P PAFKI PEELM PQ0 PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q5J RBO RHF ROL ROX ROZ TCN TEORI TLC TOX TR2 TSR VH1 WH7 WOQ X YAYTL YKOAZ YXANX ZGI ZXP --- -JH -~X 0R~ 6J9 AAPXW AARHZ AAUAY AAVAP AAYXX ABEJV ABJNI ABMNT ABXVV ACGFO ADHSS ADNWM ADQBN AEPYG AFFIJ AFGWE AFNWH AFOFC AI. AJEEA ALIPV ATGXG CITATION F9R FLUFQ FOEOM JXSIZ KBUDW KSI NOMLY OJZSN RUSNO W8F YHG ZCN ~EF ~KM |
| ID | FETCH-LOGICAL-b2556-f75074cccaea5cb7a5696c2fab2a8de193301a09c8428134ce4e3623dab7c0123 |
| ISSN | 0006-3363 |
| IngestDate | Thu Nov 21 21:46:31 EST 2024 Thu Nov 04 13:51:18 EDT 2021 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-b2556-f75074cccaea5cb7a5696c2fab2a8de193301a09c8428134ce4e3623dab7c0123 |
| OpenAccessLink | https://academic.oup.com/biolreprod/article-pdf/85/5/1025/10578510/biolreprod1025.pdf |
| PageCount | 15 |
| ParticipantIDs | crossref_primary_10_1095_biolreprod_111_091736 bioone_primary_10_1095_biolreprod_111_091736 |
| PublicationCentury | 2000 |
| PublicationDate | 20111100 2011-11-01 |
| PublicationDateYYYYMMDD | 2011-11-01 |
| PublicationDate_xml | – month: 11 year: 2011 text: 20111100 |
| PublicationDecade | 2010 |
| PublicationTitle | Biology of reproduction |
| PublicationYear | 2011 |
| Publisher | Society for the Study of Reproduction, Inc |
| Publisher_xml | – name: Society for the Study of Reproduction, Inc |
| References | ( key 20170224023818_R20) 1997; 94 ( key 20170224023818_R36) 2006; 16 ( key 20170224023818_R21) 2002; 297 ( key 20170224023818_R14) 2003 ( key 20170224023818_R04) 2007; 122 ( key 20170224023818_R18) 1986; 46 ( key 20170224023818_R24) 1997; 17 ( key 20170224023818_R15) 1987; 33 ( key 20170224023818_R28) 1998; 7 ( key 20170224023818_R03) 2004; 10 ( key 20170224023818_R09) 2008; 282 ( key 20170224023818_R30) 2002; 12 ( key 20170224023818_R16) 1981; 5 ( key 20170224023818_R22) 1990; 111 ( key 20170224023818_R35) 2008; 18 ( key 20170224023818_R26) 2007; 12 ( key 20170224023818_R01) 1998; 30 ( key 20170224023818_R08) 2001; 7 ( key 20170224023818_R02) 2005; 111 ( key 20170224023818_R27) 1994; 1 ( key 20170224023818_R13) 1986; 103 ( key 20170224023818_R17) 1998; 20 ( key 20170224023818_R12) 2009; 122 ( key 20170224023818_R34) 2008; 10 ( key 20170224023818_R05) 2005; 111 ( key 20170224023818_R19) 2002; 52 ( key 20170224023818_R37) 2005; 71 ( key 20170224023818_R23) 1998; 9 ( key 20170224023818_R31) 2008; 18 ( key 20170224023818_R33) 2009; 101 ( key 20170224023818_R07) 1989; 52 ( key 20170224023818_R38) 1999; 205 ( key 20170224023818_R40) 2004; 319 ( key 20170224023818_R06) 2004; 36 ( key 20170224023818_R11) 1978; 126 ( key 20170224023818_R39) 2002; 2 ( key 20170224023818_R10) 1984; 81 ( key 20170224023818_R25) 1986; 5 ( key 20170224023818_R32) 2004; 69 ( key 20170224023818_R29) 1995; 130 |
| References_xml | – volume: 111 start-page: 193 year: 2005 ident: key 20170224023818_R02 article-title: Frequency and distribution of chromosome abnormalities in human oocytes publication-title: Cytogenet Genome Res doi: 10.1159/000086889 – volume: 12 start-page: R12 year: 2002 ident: key 20170224023818_R30 article-title: Actomyosin rings: the riddle of the sphincter publication-title: Curr Biol doi: 10.1016/S0960-9822(01)00639-X – volume: 282 start-page: 70 year: 2008 ident: key 20170224023818_R09 article-title: Morphogenesis of the mammalian blastocyst publication-title: Mol Cell Endocrinol doi: 10.1016/j.mce.2007.11.004 – volume: 46 start-page: 1867 year: 1986 ident: key 20170224023818_R18 article-title: Altered biochemical properties of actin in normal skin fibroblasts from individuals predisposed to dominantly inherited cancers publication-title: Cancer Res – volume: 126 start-page: 783 year: 1978 ident: key 20170224023818_R11 article-title: At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide publication-title: J Mol Biol doi: 10.1016/0022-2836(78)90020-7 – volume: 69 start-page: 66 year: 2004 ident: key 20170224023818_R32 article-title: Fate of the first polar bodies in mouse oocytes publication-title: Mol Reprod Dev doi: 10.1002/mrd.20148 – volume: 5 start-page: 387 year: 1981 ident: key 20170224023818_R16 article-title: Visualization of assembled and disassembled microtubule protein by double label fluorescence microscopy publication-title: Cell Biol Int Rep doi: 10.1016/0309-1651(81)90009-6 – volume: 2 start-page: 17 year: 2002 ident: key 20170224023818_R39 article-title: Rac-1 and IQGAP are potential regulators of E-cadherin-catenin interactions during murine preimplantation development publication-title: Gene Expr Patterns doi: 10.1016/S0925-4773(02)00350-7 – volume: 36 start-page: 744 year: 2004 ident: key 20170224023818_R06 article-title: BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice publication-title: Nat Genet doi: 10.1038/ng1382 – volume: 16 start-page: 214 year: 2006 ident: key 20170224023818_R36 article-title: Cdc42 activation couples spindle positioning to first polar body formation in oocyte maturation publication-title: Curr Biol doi: 10.1016/j.cub.2005.11.067 – volume: 319 start-page: 643 year: 2004 ident: key 20170224023818_R40 article-title: Rho-kinase is involved in mouse blastocyst cavity formation publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2004.05.040 – volume: 297 start-page: 1343 year: 2002 ident: key 20170224023818_R21 article-title: Meiotic arrest in the mouse follicle maintained by a Gs protein in the oocyte publication-title: Science doi: 10.1126/science.1073978 – volume: 101 start-page: 69 year: 2009 ident: key 20170224023818_R33 article-title: Actin filaments: key players in the control of asymmetric divisions in mouse oocytes publication-title: Biol Cell doi: 10.1042/BC20080003 – volume: 7 start-page: 95 year: 1998 ident: key 20170224023818_R28 article-title: Restricted beta-galactosidase expression of a hygromycin-lacZ gene targeted to the beta-actin locus and embryonic lethality of beta-actin mutant mice publication-title: Transgenic Res doi: 10.1023/A:1008816308171 – volume: 10 start-page: 1301 year: 2008 ident: key 20170224023818_R34 article-title: Actin-driven chromosomal motility leads to symmetry breaking in mammalian meiotic oocytes publication-title: Nat Cell Biol doi: 10.1038/ncb1788 – volume: 81 start-page: 211 year: 1984 ident: key 20170224023818_R10 article-title: Changes in actin distribution during fertilization of the mouse egg publication-title: J Embryol Exp Morphol – volume: 103 start-page: 2787 year: 1986 ident: key 20170224023818_R13 article-title: A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation publication-title: J Cell Biol doi: 10.1083/jcb.103.6.2787 – volume: 33 start-page: 232 year: 1987 ident: key 20170224023818_R15 article-title: Actin-isoform pattern as a marker of normal or pathological smooth-muscle and fibroblastic tissues publication-title: Differentiation doi: 10.1111/j.1432-0436.1987.tb01562.x – volume: 111 start-page: 250 year: 2005 ident: key 20170224023818_R05 article-title: Effect of meiotic recombination on the production of aneuploid gametes in humans publication-title: Cytogenet Genome Res doi: 10.1159/000086896 – volume: 5 start-page: 343 year: 1986 ident: key 20170224023818_R25 article-title: Cytoskeletal dynamics in the mouse egg publication-title: J Cell Sci Suppl – volume-title: Manipulating the Mouse Embryo. A Laboratory Manual, 3rd ed year: 2003 ident: key 20170224023818_R14 – volume: 18 start-page: 1986 year: 2008 ident: key 20170224023818_R35 article-title: A new model for asymmetric spindle positioning in mouse oocytes publication-title: Curr Biol doi: 10.1016/j.cub.2008.11.022 – volume: 94 start-page: 9165 year: 1997 ident: key 20170224023818_R20 article-title: CENP-E is an essential kinetochore motor in maturing oocytes and is masked during mos-dependent, cell cycle arrest at metaphase II publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.94.17.9165 – volume: 17 start-page: 9565 year: 1997 ident: key 20170224023818_R24 article-title: Isoform specificity in the relationship of actin to dendritic spines publication-title: J Neurosci doi: 10.1523/JNEUROSCI.17-24-09565.1997 – volume: 111 start-page: 1491 year: 1990 ident: key 20170224023818_R22 article-title: Microinjected centromere kinetochore antibodies interfere with chromosome movement in meiotic and mitotic mouse oocytes publication-title: J Cell Biol – volume: 9 start-page: 2509 year: 1998 ident: key 20170224023818_R23 article-title: Differential expression and functions of cortical myosin IIA and IIB isotypes during meiotic maturation, fertilization, and mitosis in mouse oocytes and embryos publication-title: Mol Biol Cell doi: 10.1091/mbc.9.9.2509 – volume: 18 start-page: 1514 year: 2008 ident: key 20170224023818_R31 article-title: Spindle positioning in mouse oocytes relies on a dynamic meshwork of actin filaments publication-title: Curr Biol doi: 10.1016/j.cub.2008.08.044 – volume: 10 start-page: 401 year: 2004 ident: key 20170224023818_R03 article-title: Meiotic and mitotic nondisjunction: lessons from preimplantation genetic diagnosis publication-title: Hum Reprod Update doi: 10.1093/humupd/dmh036 – volume: 52 start-page: 170 year: 1989 ident: key 20170224023818_R07 article-title: Nocodazole sensitivity, age-related aneuploidy, and alterations in the cell cycle during maturation of mouse oocytes publication-title: Cytogenet Cell Genet doi: 10.1159/000132871 – volume: 12 start-page: 309 year: 2007 ident: key 20170224023818_R26 article-title: Rac activity is polarized and regulates meiotic spindle stability and anchoring in mammalian oocytes publication-title: Dev Cell doi: 10.1016/j.devcel.2006.12.010 – volume: 30 start-page: 143 year: 1998 ident: key 20170224023818_R01 article-title: Genetics of oocyte ageing publication-title: Maturitas doi: 10.1016/S0378-5122(98)00070-X – volume: 7 start-page: 49 year: 2001 ident: key 20170224023818_R08 article-title: Association between spindle assembly checkpoint expression and maternal age in human oocytes publication-title: Mol Hum Reprod doi: 10.1093/molehr/7.1.49 – volume: 52 start-page: 183 year: 2002 ident: key 20170224023818_R19 article-title: Arrest of cell cycle progression during first interphase in murine zygotes microinjected with anti-PCM-1 antibodies publication-title: Cell Motil Cytoskeleton doi: 10.1002/cm.10043 – volume: 130 start-page: 887 year: 1995 ident: key 20170224023818_R29 article-title: The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo publication-title: J Cell Biol doi: 10.1083/jcb.130.4.887 – volume: 122 start-page: 62 year: 2007 ident: key 20170224023818_R04 article-title: The National Down Syndrome Project: design and implementation publication-title: Public Health Rep doi: 10.1177/003335490712200109 – volume: 122 start-page: 2980 year: 2009 ident: key 20170224023818_R12 article-title: Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity publication-title: J Cell Sci doi: 10.1242/jcs.041970 – volume: 205 start-page: 322 year: 1999 ident: key 20170224023818_R38 article-title: A role for Rho-like GTPases in the polarisation of mouse eight-cell blastomeres publication-title: Dev Biol doi: 10.1006/dbio.1998.9117 – volume: 20 start-page: 847 year: 1998 ident: key 20170224023818_R17 article-title: Rapid actin-based plasticity in dendritic spines publication-title: Neuron doi: 10.1016/S0896-6273(00)80467-5 – volume: 71 start-page: 256 year: 2005 ident: key 20170224023818_R37 article-title: Small GTPase RhoA is required for ooplasmic segregation and spindle rotation, but not for spindle organization and chromosome separation during mouse oocyte maturation, fertilization, and early cleavage publication-title: Mol Reprod Dev doi: 10.1002/mrd.20253 – volume: 1 start-page: 1 year: 1994 ident: key 20170224023818_R27 article-title: Actin publication-title: Protein Profile |
| SSID | ssj0014323 |
| Score | 2.0462732 |
| Snippet | In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning... |
| SourceID | crossref bioone |
| SourceType | Aggregation Database Publisher |
| StartPage | 1025 |
| SubjectTerms | actin actin isoforms asymmetric cell division blastocyst meiosis oocyte polarity preimplantation development preimplantation embryo |
| Title | Beta- and Gamma-Cytoplasmic Actins Are Required for Meiosis in Mouse Oocytes1 |
| URI | http://www.bioone.org/doi/abs/10.1095/biolreprod.111.091736 |
| Volume | 85 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELaWIiQuFW1BtDzkA-JSvGA7j-a4LIUFtCBBK_W28ivSCpJU2-xhufHPmbGd3VTdQ8sliizFSjxf7M8zn2cIeVUkIs8Sa5hRImeJKC3TXBmmrSwN184Kn7tz-i2bnCdfLtKLweBv_3RJq4fmz9ZzJf9jVWgDu-Ip2TtYdt0pNMA92BeuYGG43srG712rmHf_f1JVpdh41TaXQIdR7z6Ciay-Oh4tsDADyn2BWaKkcOrmDSYhmdfwPy-v3PH3xqyAb_Jr4d2YmgmYJGa99ElhewF72LubX1UsrzwOsfbfvQD9xCtFg3MexZrVhi5jGQivrXWLjUbIF3QJLoIlng-LPdm1d5Vfc0b0labIm392ibF_9N71hsgTl0wmZZzoXJyIRcFyEUrudDN1KO4TEZn2pl1gSWlvCcfw9tblAfgk2BTTW4XBwwVjCIwpl9k9ch9zKWL5hQ-fv64DUYkUsRhfeMXuEFiRvt3aDRAZaG9q1-M5PcJy9ojsxp0GHQXY7JGBq_fJwahWbVOt6Gvqtb_ezPvkQTT4AZl6TFHAFL2BKRowBV062mGKghFoxBSd19RjinaYekzOP56ejScsltxgGnPRsRIIZJ4Y-K2dSo3OVZoVmRGl0kKdWMfR_cXVu8KcwLaVy8S4xAEFklbp3CA9f0J2avj6p4Qq2EyU2hoj0zKxvNAuw8xRVjrOjRT6kLwJIzW7DHlVZkEPkc42A4v701kY2EMy7Mbzdg8c3a3_Z-ThBtDPyU67WLoXwDdb_dIj4h88goIi |
| link.rule.ids | 314,780,784,27924,27925 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Beta-+and+Gamma-Cytoplasmic+Actins+Are+Required+for+Meiosis+in+Mouse+Oocytes1&rft.jtitle=Biology+of+reproduction&rft.au=Brockmann%2C+C%C3%A9line&rft.au=Huarte%2C+Joachim&rft.au=Dugina%2C+Vera&rft.au=Challet%2C+Ludivine&rft.date=2011-11-01&rft.pub=Society+for+the+Study+of+Reproduction%2C+Inc&rft.issn=0006-3363&rft.eissn=1529-7268&rft.volume=85&rft.issue=5&rft.spage=1025&rft.epage=1039&rft_id=info:doi/10.1095%2Fbiolreprod.111.091736 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-3363&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-3363&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-3363&client=summon |