Acrosomal Swelling Is Triggered by cAMP Downstream of the Opening of Store-Operated Calcium Channels During Acrosomal Exocytosis in Human Sperm1
Acrosomal exocytosis in mammalian sperm is a regulated secretion with unusual characteristics. One of its most striking features is the postfusion loss of the outer acrosomal membrane and the overlying plasma membrane as hybrid vesicles. We have previously reported in human sperm that, by preventing...
Saved in:
| Published in | Biology of reproduction Vol. 94; no. 3 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Society for the Study of Reproduction, Inc
01.03.2016
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Acrosomal exocytosis in mammalian sperm is a regulated secretion with unusual characteristics. One of its most striking features is the postfusion loss of the outer acrosomal membrane and the overlying plasma membrane as hybrid vesicles. We have previously reported in human sperm that, by preventing the release of calcium from the acrosome, the exocytic process can be arrested at a stage where the acrosomes are profusely swollen, with invaginations of the outer acrosomal membrane. In this report, we show by transmission electron microcopy swelling with similar characteristics without arresting the exocytic process. Acrosomal swelling was observed when secretion was promoted by pharmacological and physiological inducers of the acrosome reaction that trigger exocytosis by different mechanisms. We show that progesterone- and thapsigargin-induced swelling depended on a calcium influx from the extracellular medium through store-operated calcium channels. However, calcium was dispensable when sperm were stimulated with cAMP analogs. KH7, an inhibitor of the soluble adenylyl cyclase, blocked progesterone-induced swelling. Our results indicate that swelling is a required process for acrosomal exocytosis triggered by activation of an adenylyl cyclase downstream of the opening of store-operated calcium channels. |
|---|---|
| AbstractList | Acrosomal exocytosis in mammalian sperm is a regulated secretion with unusual characteristics. One of its most striking features is the postfusion loss of the outer acrosomal membrane and the overlying plasma membrane as hybrid vesicles. We have previously reported in human sperm that, by preventing the release of calcium from the acrosome, the exocytic process can be arrested at a stage where the acrosomes are profusely swollen, with invaginations of the outer acrosomal membrane. In this report, we show by transmission electron microcopy swelling with similar characteristics without arresting the exocytic process. Acrosomal swelling was observed when secretion was promoted by pharmacological and physiological inducers of the acrosome reaction that trigger exocytosis by different mechanisms. We show that progesterone- and thapsigargin-induced swelling depended on a calcium influx from the extracellular medium through store-operated calcium channels. However, calcium was dispensable when sperm were stimulated with cAMP analogs. KH7, an inhibitor of the soluble adenylyl cyclase, blocked progesterone-induced swelling. Our results indicate that swelling is a required process for acrosomal exocytosis triggered by activation of an adenylyl cyclase downstream of the opening of store-operated calcium channels. |
| Author | Zanetti, M. Natalia Mayorga, Luis S Pocognoni, Cristian A Sosa, Claudia M |
| Author_xml | – sequence: 1 givenname: Claudia M surname: Sosa fullname: Sosa, Claudia M – sequence: 2 givenname: M. Natalia surname: Zanetti fullname: Zanetti, M. Natalia – sequence: 3 givenname: Cristian A surname: Pocognoni fullname: Pocognoni, Cristian A – sequence: 4 givenname: Luis S surname: Mayorga fullname: Mayorga, Luis S |
| BookMark | eNqNkd1uwjAMhaOJSQO2R5iUFyhLmro_lwjYQGJiEuy6ShsXOrUJSooYb7FHXiomcbsry_b5bOt4RAbaaCTkmbMJZxm8FLVpLB6tURPOYcKFCAW_I0MOYRYkYZwOyJAxFgdCxOKBjJz7YoxHXjUkP9PSGmda2dDtGZum1nu6cnRn6_0eLSpaXGg5ff-gc3PWrrMoW2oq2h2Qbo6oe7lPt52xGPiClZ1nZrIp61NLZwepNTaOzk-2V952Lb5NeemMqx2tNV2eWqnp1uMtfyT3lWwcPv3FMfl8Xexmy2C9eVvNpuugCAF4kIAMJcYhKsUqmUUxJEJFRZHGoLKkAKkigVFaQMlAIfIyhUgliWQxgIAoFWMC17n9Tc5ilR9t3Up7yTnLe1vzm625tzW_2uo5ceV827_hn9QvoQCDNw |
| CitedBy_id | crossref_primary_10_3389_fcell_2023_1148831 crossref_primary_10_7554_eLife_69160 crossref_primary_10_1093_biolre_ioac136 crossref_primary_10_3389_fphys_2021_682790 crossref_primary_10_1016_j_bbamcr_2020_118704 crossref_primary_10_1016_j_mce_2020_110952 crossref_primary_10_1134_S1062360420040037 crossref_primary_10_1093_molehr_gaad015 crossref_primary_10_1093_molehr_gaac005 crossref_primary_10_1016_j_bbrc_2018_02_042 crossref_primary_10_1111_and_13742 crossref_primary_10_3390_ijms232315068 crossref_primary_10_1186_s40850_020_00064_4 crossref_primary_10_1111_andr_12467 crossref_primary_10_1126_sciadv_ade7607 crossref_primary_10_1124_pr_116_013078 crossref_primary_10_1016_j_xfss_2023_05_001 crossref_primary_10_1093_humupd_dmw014 crossref_primary_10_1016_j_pharmthera_2018_05_008 |
| Cites_doi | 10.1242/jcs.02297 10.1007/978-1-4939-2569-8_12 10.1124/jpet.113.208496 10.1091/mbc.11.5.1571 10.1371/journal.pbio.0030323 10.1172/JCI41585 10.1080/15216540701222872 10.1074/jbc.M508854200 10.1042/BJ20141169 10.1074/jbc.M208587200 10.1007/s00441-012-1397-5 10.1530/jrf.0.0950755 10.1093/molehr/7.2.119 10.1016/j.pharmthera.2010.04.016 10.1074/jbc.M302179200 10.1083/jcb.34.3.C1 10.1002/jez.1402670312 10.1152/physrev.00028.2010 10.1096/fj.06-7716com 10.1002/mrd.1120200103 10.1038/emboj.2012.30 10.1530/REP-09-0134 10.1016/j.bbrc.2007.05.061 10.1093/humrep/det454 10.1095/biolreprod.109.076166 10.1042/BJ20120339 10.1074/jbc.M113.489476 10.1530/REP-15-0102 10.1093/molehr/gav019 10.1242/jcs.81.1.43 10.1074/jbc.M700854200 10.1093/biolreprod/3.2.180 10.1074/jbc.M109.015362 10.1074/jbc.M114.629006 10.1242/jcs.32.1.165 10.1016/S0015-0282(16)44121-X 10.1093/molehr/gau110 10.1016/j.devcel.2005.06.007 10.1074/jbc.M112.439356 |
| ContentType | Journal Article |
| Copyright | 2016 by the Society for the Study of Reproduction, Inc. This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0 |
| Copyright_xml | – notice: 2016 by the Society for the Study of Reproduction, Inc. This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0 |
| DBID | AAYXX CITATION |
| DOI | 10.1095/biolreprod.115.133231 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Biology |
| DocumentTitleAlternate | ACROSOMAL SWELLING DURING EXOCYTOSIS |
| EISSN | 1529-7268 |
| ExternalDocumentID | 10_1095_biolreprod_115_133231 |
| GroupedDBID | - 08R 0R 186 1TH 23N 2WC 3O- 3V. 48X 53G 5GY 5RE 5VS 5WD 7X7 88E 8FI 8FJ AABJS AABMN AACFU AAIMJ AAJQQ AAPBV AAPPN AAPQZ AAPSS AAUQX ABFLS ABPTD ABSAR ABSGY ABUWG ACFRR ACGFS ACNCT ACUFI ACUTJ ADBBV ADEIU ADGZP ADHKW ADIPN ADOYD ADRTK ADVEK AEDJY AELNO AELWJ AEMDU AENEX AENZO AETBJ AEWNT AFFNX AFFZL AFKRA AFULF AFXEN AGINJ AHMBA AIKOY AKPMI ALMA_UNASSIGNED_HOLDINGS APIBT ARIXL ASAOO ATDFG AYOIW AZQFJ BAWUL BAYMD BBAFP BBNVY BCRHZ BENPR BEYMZ BHONS BHPHI BPHCQ BSWAC BVXVI BYORX C1A C45 CAG CDBKE COF CS3 DAKXR DC7 DIK DPPUQ DU5 E3Z EBS EF EJD F5P FA8 FHSFR FYUFA GAUVT GJ GJXCC H13 HCIFZ H~9 IAO IHR INH INIJC JH KM KOP KQ8 KSN M1P M7P MBTAY MVM NLBLG O9- OBOKY ODMLO OHT OK1 OVD OWPYF P2P PAFKI PEELM PQ0 PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q5J RBO RHF ROL ROX ROZ TCN TEORI TLC TOX TR2 TSR VH1 WH7 WOQ X YAYTL YKOAZ YXANX ZGI ZXP --- -JH -~X 0R~ 6J9 AAPXW AARHZ AASNB AAUAY AAVAP AAYXX ABJNI ABMNT ABXVV ACGFO ADHSS ADNWM ADQBN AEPYG AFFIJ AFGWE AFNWH AFOFC AJEEA ALIPV ATGXG CITATION F9R FLUFQ FOEOM JXSIZ KBUDW KSI NOMLY OJZSN RUSNO W8F YHG ZCN ~EF ~KM |
| ID | FETCH-LOGICAL-b2551-75a2ae62edd0fa946573d4bb865d97b5ad43e48b5c05dee1c854d77a065535483 |
| ISSN | 0006-3363 |
| IngestDate | Thu Sep 12 19:39:29 EDT 2024 Thu Nov 04 13:51:20 EDT 2021 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-b2551-75a2ae62edd0fa946573d4bb865d97b5ad43e48b5c05dee1c854d77a065535483 |
| OpenAccessLink | https://academic.oup.com/biolreprod/article-pdf/94/3/57, 1-9/10555491/biolreprod0057.pdf |
| ParticipantIDs | crossref_primary_10_1095_biolreprod_115_133231 bioone_primary_10_1095_biolreprod_115_133231 |
| PublicationCentury | 2000 |
| PublicationDate | 20160300 2016-03-01 |
| PublicationDateYYYYMMDD | 2016-03-01 |
| PublicationDate_xml | – month: 3 year: 2016 text: 20160300 |
| PublicationDecade | 2010 |
| PublicationTitle | Biology of reproduction |
| PublicationYear | 2016 |
| Publisher | Society for the Study of Reproduction, Inc |
| Publisher_xml | – name: Society for the Study of Reproduction, Inc |
| References | ( key 20170224025532_CIT0037) 2015; 21 ( key 20170224025532_CIT0026) 1992; 95 ( key 20170224025532_CIT0027) 2002; 277 ( key 20170224025532_CIT0002) 2010; 120 ( key 20170224025532_CIT0010) 2007; 59 ( key 20170224025532_CIT0024) 2015; 21 ( key 20170224025532_CIT0021) 1979; 32 ( key 20170224025532_CIT0023) 2015; 93 ( key 20170224025532_CIT0016) 1988; 20 ( key 20170224025532_CIT0007) 2012; 349 ( key 20170224025532_CIT0033) 2006; 281 ( key 20170224025532_CIT0034) 2015; 150 ( key 20170224025532_CIT0042) 2013; 288 ( key 20170224025532_CIT0003) 2012; 31 ( key 20170224025532_CIT0008) 2012; 448 ( key 20170224025532_CIT0028) 2005; 118 ( key 20170224025532_CIT0015) 2007; 282 ( key 20170224025532_CIT0043) 2013; 347 ( key 20170224025532_CIT0005) 2009; 138 ( key 20170224025532_CIT0001) 1994 ( key 20170224025532_CIT0011) 2015; 465 ( key 20170224025532_CIT0030) 2000; 11 ( key 20170224025532_CIT0019) 1970; 3 ( key 20170224025532_CIT0022) 2009; 81 ( key 20170224025532_CIT0004) 2013; 288 ( key 20170224025532_CIT0041) 2005; 9 ( key 20170224025532_CIT0032) 2009; 284 ( key 20170224025532_CIT0031) 1993; 267 ( key 20170224025532_CIT0006) 2011; 91 ( key 20170224025532_CIT0025) 2007; 21 ( key 20170224025532_CIT0029) 2001; 7 ( key 20170224025532_CIT0038) 2003; 278 ( key 20170224025532_CIT0018) 1978; 32 ( key 20170224025532_CIT0020) 1967; 34 ( key 20170224025532_CIT0017) 1986; 81 ( key 20170224025532_CIT0035) 2010; 127 ( key 20170224025532_CIT0009) 2014; 91 ( key 20170224025532_CIT0036) 2014; 29 ( key 20170224025532_CIT0039) 2015; 290 ( key 20170224025532_CIT0012) 2015; 1298 ( key 20170224025532_CIT0014) 2005; 3 ( key 20170224025532_CIT0040) 2007; 358 ( key 20170224025532_CIT0013) 2014; 12 |
| References_xml | – volume: 118 start-page: 1673 year: 2005 ident: key 20170224025532_CIT0028 article-title: Secretory pathway Ca2+-ATPase (SPCA1) Ca2+ pumps, not SERCAs, regulate complex [Ca2+](i) signals in human spermatozoa publication-title: J Cell Sci doi: 10.1242/jcs.02297 – volume: 1298 start-page: 141 year: 2015 ident: key 20170224025532_CIT0012 article-title: Small GTPases in acrosomal exocytosis publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-2569-8_12 – volume: 347 start-page: 589 year: 2013 ident: key 20170224025532_CIT0043 article-title: Pharmacological distinction between soluble and transmembrane adenylyl cyclases publication-title: J Pharmacol Exp Ther doi: 10.1124/jpet.113.208496 – volume: 11 start-page: 1571 year: 2000 ident: key 20170224025532_CIT0030 article-title: Ca2+ entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction publication-title: Mol Biol Cell doi: 10.1091/mbc.11.5.1571 – volume: 3 start-page: e323 year: 2005 ident: key 20170224025532_CIT0014 article-title: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis publication-title: PLoS Biology doi: 10.1371/journal.pbio.0030323 – volume: 120 start-page: 984 year: 2010 ident: key 20170224025532_CIT0002 article-title: Fertilization: a sperm’s journey to and interaction with the oocyte publication-title: J Clin Invest doi: 10.1172/JCI41585 – volume: 59 start-page: 286 year: 2007 ident: key 20170224025532_CIT0010 article-title: Acrosomal exocytosis, a special type of regulated secretion publication-title: IUBMB Life doi: 10.1080/15216540701222872 – volume: 281 start-page: 8656 year: 2006 ident: key 20170224025532_CIT0033 article-title: Calcium-induced acrosomal exocytosis requires cAMP acting through a protein kinase A-independent, Epac-mediated pathway publication-title: J Biol Chem doi: 10.1074/jbc.M508854200 – volume: 465 start-page: 359 year: 2015 ident: key 20170224025532_CIT0011 article-title: The proteins of exocytosis: lessons from the sperm model publication-title: Biochem J doi: 10.1042/BJ20141169 – volume: 277 start-page: 49326 year: 2002 ident: key 20170224025532_CIT0027 article-title: The intraacrosomal calcium pool plays a direct role in acrosomal exocytosis publication-title: J Biol Chem doi: 10.1074/jbc.M208587200 – volume: 349 start-page: 749 year: 2012 ident: key 20170224025532_CIT0007 article-title: Are TRP channels involved in sperm development and function? publication-title: Cell Tissue Res doi: 10.1007/s00441-012-1397-5 – volume: 95 start-page: 755 year: 1992 ident: key 20170224025532_CIT0026 article-title: Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisum sativum agglutinin publication-title: J Reprod Fertil doi: 10.1530/jrf.0.0950755 – volume: 7 start-page: 119 year: 2001 ident: key 20170224025532_CIT0029 article-title: Intracellular calcium store depletion and acrosome reaction in human spermatozoa: role of calcium and plasma membrane potential publication-title: Mol Hum Reprod doi: 10.1093/molehr/7.2.119 – volume: 127 start-page: 121 year: 2010 ident: key 20170224025532_CIT0035 article-title: ORAI-mediated calcium entry: mechanism and roles, diseases and pharmacology publication-title: Pharmacol Ther doi: 10.1016/j.pharmthera.2010.04.016 – volume: 278 start-page: 35394 year: 2003 ident: key 20170224025532_CIT0038 article-title: cAMP analog mapping of Epac1 and cAMP kinase. Discriminating analogs demonstrate that Epac and cAMP kinase act synergistically to promote PC-12 cell neurite extension publication-title: J Biol Chem doi: 10.1074/jbc.M302179200 – volume: 12 start-page: 43 year: 2014 ident: key 20170224025532_CIT0013 article-title: Epac, Rap and Rab3 act in concert to mobilize calcium from sperm’s acrosome during exocytosis publication-title: Cell Commun Signal – volume: 34 start-page: C1 year: 1967 ident: key 20170224025532_CIT0020 article-title: Membrane vesiculation as a feature of the mammalian acrosome reaction publication-title: J Cell Biol doi: 10.1083/jcb.34.3.C1 – volume: 267 start-page: 350 year: 1993 ident: key 20170224025532_CIT0031 article-title: Initiation of the human sperm acrosome reaction by thapsigargin publication-title: J Exp Zool doi: 10.1002/jez.1402670312 – volume: 91 start-page: 1305 year: 2011 ident: key 20170224025532_CIT0006 article-title: Calcium channels in the development, maturation, and function of spermatozoa publication-title: Physiol Rev doi: 10.1152/physrev.00028.2010 – volume: 21 start-page: 4121 year: 2007 ident: key 20170224025532_CIT0025 article-title: Membrane-permeant Rab3A triggers acrosomal exocytosis in living human sperm publication-title: FASEB J doi: 10.1096/fj.06-7716com – volume: 20 start-page: 11 year: 1988 ident: key 20170224025532_CIT0016 article-title: Ultrastructural studies of the early events of the human sperm acrosome reaction as initiated by human follicular fluid publication-title: Gamete Res doi: 10.1002/mrd.1120200103 – volume: 31 start-page: 1654 year: 2012 ident: key 20170224025532_CIT0003 article-title: The CatSper channel: a polymodal chemosensor in human sperm publication-title: EMBO J doi: 10.1038/emboj.2012.30 – volume: 138 start-page: 425 year: 2009 ident: key 20170224025532_CIT0005 article-title: Ca2+-stores in sperm: their identities and functions publication-title: Reproduction doi: 10.1530/REP-09-0134 – volume: 358 start-page: 1128 year: 2007 ident: key 20170224025532_CIT0040 article-title: Particulate and soluble adenylyl cyclases participate in the sperm acrosome reaction publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2007.05.061 – volume: 29 start-page: 418 year: 2014 ident: key 20170224025532_CIT0036 article-title: The CatSper calcium channel in human sperm: relation with motility and involvement in progesterone-induced acrosome reaction publication-title: Hum Reprod doi: 10.1093/humrep/det454 – volume: 91 start-page: 67 year: 2014 ident: key 20170224025532_CIT0009 article-title: Acrosome reaction and Ca2+ imaging in single human spermatozoa: new regulatory roles of [Ca2+]i publication-title: Biol Reprod – volume: 81 start-page: 396 year: 2009 ident: key 20170224025532_CIT0022 article-title: Acrosomal swelling and membrane docking are required for hybrid vesicle formation during the human sperm acrosome reaction publication-title: Biol Reprod doi: 10.1095/biolreprod.109.076166 – volume: 448 start-page: 189 year: 2012 ident: key 20170224025532_CIT0008 article-title: 2-APB-potentiated channels amplify CatSper-induced Ca2+ signals in human sperm publication-title: Biochem J doi: 10.1042/BJ20120339 – start-page: 189 volume-title: The Physiology of Reproduction, 2nd ed year: 1994 ident: key 20170224025532_CIT0001 article-title: Mammalian fertilization – volume: 288 start-page: 35307 year: 2013 ident: key 20170224025532_CIT0042 article-title: Compartmentalization of distinct cAMP signaling pathways in mammalian sperm publication-title: J Biol Chem doi: 10.1074/jbc.M113.489476 – volume: 150 start-page: R65 year: 2015 ident: key 20170224025532_CIT0034 article-title: Regulation and roles of Ca2+ stores in human sperm publication-title: Reproduction doi: 10.1530/REP-15-0102 – volume: 21 start-page: 563 year: 2015 ident: key 20170224025532_CIT0037 article-title: Cell-penetrating peptides, targeting the regulation of store-operated channels, slow decay of the progesterone-induced [Ca2+]i signal in human sperm publication-title: Mol Hum Reprod doi: 10.1093/molehr/gav019 – volume: 81 start-page: 43 year: 1986 ident: key 20170224025532_CIT0017 article-title: Membrane fusion events in the Ca2+/ionophore-induced acrosome reaction of ram spermatozoa publication-title: J Cell Sci doi: 10.1242/jcs.81.1.43 – volume: 282 start-page: 26335 year: 2007 ident: key 20170224025532_CIT0015 article-title: Complexin/synaptotagmin interplay controls acrosomal exocytosis publication-title: J Biol Chem doi: 10.1074/jbc.M700854200 – volume: 3 start-page: 180 year: 1970 ident: key 20170224025532_CIT0019 article-title: The acrosomal region and the acrosome reaction in sperm of the golden hamster publication-title: Biol Reprod doi: 10.1093/biolreprod/3.2.180 – volume: 93 start-page: 124 year: 2015 ident: key 20170224025532_CIT0023 article-title: (endosomal sorting complex required for transport) machinery is essential for acrosomal exocytosis in human sperm publication-title: Biol Reprod – volume: 284 start-page: 24825 year: 2009 ident: key 20170224025532_CIT0032 article-title: Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis publication-title: J Biol Chem doi: 10.1074/jbc.M109.015362 – volume: 290 start-page: 9823 year: 2015 ident: key 20170224025532_CIT0039 article-title: ADP ribosylation factor 6 (ARF6) promotes acrosomal exocytosis by modulating lipid turnover and Rab3A activation publication-title: J Biol Chem doi: 10.1074/jbc.M114.629006 – volume: 32 start-page: 165 year: 1978 ident: key 20170224025532_CIT0018 article-title: The osmotic properties of the acrosome of guinea-pig sperm publication-title: J Cell Sci doi: 10.1242/jcs.32.1.165 – volume: 32 start-page: 87 year: 1979 ident: key 20170224025532_CIT0021 article-title: Morphologic characteristics of the chemically induced acrosome reaction in human spermatozoa publication-title: Fertil Steril doi: 10.1016/S0015-0282(16)44121-X – volume: 21 start-page: 244 year: 2015 ident: key 20170224025532_CIT0024 article-title: Kinetics of human sperm acrosomal exocytosis publication-title: Mol Hum Reprod doi: 10.1093/molehr/gau110 – volume: 9 start-page: 249 year: 2005 ident: key 20170224025532_CIT0041 article-title: The “soluble” adenylyl cyclase in sperm mediates multiple signaling events required for fertilization publication-title: Dev Cell doi: 10.1016/j.devcel.2005.06.007 – volume: 288 start-page: 6248 year: 2013 ident: key 20170224025532_CIT0004 article-title: Ca2+ signals generated by CatSper and Ca2+ stores regulate different behaviors in human sperm publication-title: J Biol Chem doi: 10.1074/jbc.M112.439356 |
| SSID | ssj0014323 |
| Score | 2.2096257 |
| Snippet | Acrosomal exocytosis in mammalian sperm is a regulated secretion with unusual characteristics. One of its most striking features is the postfusion loss of the... |
| SourceID | crossref bioone |
| SourceType | Aggregation Database Publisher |
| SubjectTerms | acrosomal swelling acrosome reaction calcium cAMP human sperm regulated exocytosis secretory granules |
| Title | Acrosomal Swelling Is Triggered by cAMP Downstream of the Opening of Store-Operated Calcium Channels During Acrosomal Exocytosis in Human Sperm1 |
| URI | http://www.bioone.org/doi/abs/10.1095/biolreprod.115.133231 |
| Volume | 94 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1fb9MwELfKEBIvE2wgNv7ID4iXktAkdtI8RmNogIqQ2kl7q2zHQZWWBNFUUD4F34ivxp3t_Cmq0MZL1LqyneR-9d3Zd78j5GU6KSZhqpgXKsk8xpj0RJJIj6WFioI8UanE3OHZp_jikn244lej0e9B1NKmkb76uTev5H-kCm0gV8ySvYVku0GhAT6DfOEKEobrjWScoY6rSyT0wD04k56yHi_A4f6CJTjRtFTZ7DNYyd-RKFaLsg0JwDgSF-88B69be9CApBE5Jmup1aY0aQcVKM7xW5vI2M91_qNW26ZGJhMkyjKnAHPoXgY7J8SO3QlmQOJMwys7OPOf12tjtp5diw1AdDzzuy1sUenGBhnMfAymxp2YbgWvMd6pNnWosCSZXaCyrvNMbLFOldlt2MANzv3htkYQ93Fd3VIde1HkVj_tVucw9ZLQ1uFpl29bI9nBNNqrFcCMBFEiq5V9YNAT3AfnPHQKaIeF-y_t2MUs2tN6vuyHAe-JL-0wd8jdEJkG0Sp__7E7xmJR6Er52WdpU8hS_mbv3YAZBO11pQdW0sDcWTwgh85PoZkF3UMy0tUROc4q0dTllr6iJnLYSPiI3HOyPia_OpTQFpF0taYdIqncUkQk7RFJ64ICIqlDJH7dRSR1iKQtIqlFJO3n6hFJVxU1iKQWkY_I5bvzxdmF52p-eBKc28BLuAiFjkOd55NCpCzmSZQzKacxz9NEcpGzSLOp5GrCc60DNeUsTxIBljSPwPuOHpMDQKF-QijPg7TQ8IuQgiUqAsdegLchp4WQ6bQIT8hr-7KXXy2xy_KfIj4hfiuSm3U4vW2Hp-R-_094Rg6abxv9HEzeRr4wsPoDF6uxjA |
| link.rule.ids | 315,783,787,27937,27938 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Acrosomal+Swelling+is+Triggered+by+cAMP+Downstream+of+the+Opening+of+Store-Operated+Calcium+Channels+During+Acrosomal+Exocytosis+in+Human+Sperm1&rft.jtitle=Biology+of+reproduction&rft.au=Sosa%2C+Claudia+M.&rft.au=Zanetti%2C+M.+Natalia&rft.au=Pocognoni%2C+Cristian+A.&rft.au=Mayorga%2C+Luis+S.&rft.date=2016-03-01&rft.issn=0006-3363&rft.eissn=1529-7268&rft.volume=94&rft.issue=3&rft_id=info:doi/10.1095%2Fbiolreprod.115.133231&rft.externalDBID=n%2Fa&rft.externalDocID=10_1095_biolreprod_115_133231 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-3363&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-3363&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-3363&client=summon |